Part:BBa_K1073024
Constitutively expressed chromoprotein amilGFP
The device consists of a strong promoter (BBa_J23100) combined with a RBS (BBa_B0032) and the encoding region of the chromoprotein amilGFP. This results in a constitutively high expression of amilGFP.
Usage and Biology
During cultivation, the chromoprotein amilGFP is expressed and stored intracellularly. The yellow color of the protein can be seen by the naked eye, thus the chromoprotein can be used as a reporter or selection marker to distinguish cells easily.
iGEM Team Braunschweig 2013:
Left: Liquid cultures of E. coli XL1 expressing chromoproteins amilGFP , eforRed (BBa_K1073022) and aeBlue (BBa_K1073020).
Right: Cell pellet of E. coli XL1 expressing amilGFP.
The yellow color of amilGFP cannot only be seen by the naked eye but does also fluoresce when excited. Thus it can be used to identify cells with fluorescence microscopy or flow cytometry. For further information as well as absorption and emission spectra see experience page.
iGEM Team Braunschweig 2013: Supernatant after cell disruption containing chromoproteins of eforRed (BBa_K1073022) and amilGFP. Both chromoproteins fluoresce when excited by UV-light.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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