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DarR

Part:BBa_K1045017:Design

Designed by: iGEM Team Göttingen 2013   Group: iGEM13_Goettingen   (2013-09-20)
Revision as of 17:23, 4 October 2013 by Kati (Talk | contribs) (→‎Source)

DarR reporter system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 806
    Illegal NheI site found at 829
    Illegal NheI site found at 909
    Illegal NheI site found at 932
    Illegal NotI site found at 718
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 731
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 860
    Illegal AgeI site found at 168
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1630


Design Notes

DarR fused to the terminator were cut out from BBa_K1045016 and ligated in a pre-fixing composition into BBa_K1045015.

Source

This composite part was constructed using parts which were either obtained via hybridization oligos (BBa_1045011;BBa_1045012)or by amplification of plasmid DNA (BBa_1045009) or M. smegmatis chromosomal DNA (BBa_1045001).

References