Part:BBa_K801060:Design

(+)-Limonene synthase 1 with Strep-tag and yeast consensus sequence.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1683
  Illegal SapI.rc site found at 107
  

Keywords: limonene synthase, limonene

Abbreviations:

Design Notes

Related BioBrick:

• BBa_K801061: (+)-Limonene synthase 1 coding region
• BBa_K801062: (+)-limonene synthase 1 expression cassette for yeast
• BBa_K801063: (+)-limonene synthase 1 expression cassette for yeast
• BBa_K801064: (+)-limonene synthase 1 expression cassette for yeast
• BBa_K801065: (+)-limonene synthase 1 with Strep-Tag
• BBa_K801066: (+)-limonene synthase 1 with Yeast Consensus Sequence for improved expression

Quality control measures:

• Biobrick was sequenced

Backbone:

• Backbone name: pSB1C3
• Resistance: Cp
• Copynumber: high

Protein coding:

• BioBrick encodes (+)-limonene synthase
• UniProt entry: [http://www.uniprot.org/uniprot/Q8L5K3 Q8L5K3]

Enzymatic activity:

• E.C. Number: [http://enzyme.expasy.org/EC/4.2.3.20 4.2.3.20]

Cytotoxicity:

• see toxicity assay

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

• BioBrick: BBa_I742111

• Forward Primer: TUM12-LIMS1-fw-cons: 5'- GGATCCTCTAGAGTACACAATGCGTCGTTCAGCAAAC - 3'
• Reverse Primer: TUM12-LIMS1-rv: 5'- GGATCCACCGGTGCCTTTGGTGCCAGGAGA - 3'

Organism:

• Gene sequence derived from Citrus limon

References

Literature references:

• [http://www.ncbi.nlm.nih.gov/pubmed/12084056Pubmed: Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A., 2002: Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases. Eur J Biochem, 269(13):3160–71.]

Database references:

• [http://www.ncbi.nlm.nih.gov/nuccore/21435702 GenBank: source of the sequence]
• [http://www.uniprot.org/uniprot/Q8L5K3 Uniprot: (R)-limonene synthase 1]