Difference between revisions of "Part:BBa K323135:Design"

(→‎Source)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Part BBa_K323134 was ligated into a vector containing part BBa_K323085 as a back insert. The resulting fragment was then ligated into the ''Bsa''I cloning site of part BBa_K323110.
+
[[Part:BBa_K323134]] was ligated into a vector containing [[Part:BBa_K323085]] as a back insert. The resulting fragment was then ligated into the ''Bsa''I cloning site of [[Part:BBa_K323110]].
  
 
===Source===
 
===Source===

Revision as of 21:16, 23 October 2010

VioA and VioB enzymes fused with zinc fingers under pBAD promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1204
    Illegal NheI site found at 2568
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 6132
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5657
    Illegal AgeI site found at 979
    Illegal AgeI site found at 5853
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

Part:BBa_K323134 was ligated into a vector containing Part:BBa_K323085 as a back insert. The resulting fragment was then ligated into the BsaI cloning site of Part:BBa_K323110.

Source

The part was designed from three parts (BBaK_323110, BBa_K323085 and BBaK_323134) that our team previously constructed.

References