Difference between revisions of "Part:BBa K325219:ArabinosetoLight"
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| − | [[Image:Arabinose_activation.png|thumb|569px|center|'''Figure 1 - Transfer function of <partinfo>K325219</partinfo>. The data points represent the mean of 3 individual measurements. The corresponding error bars represent an interval of twice the standard deviation centred around the mean value.''']] | + | [[Image:Arabinose_activation.png|thumb|569px|center|'''Figure 1 - Transfer function of <partinfo>K325219</partinfo>. The data points represent the mean of 3 individual measurements. The corresponding error bars represent an interval of twice the standard deviation centred around the mean value. The values were obtained by integrating the light output obtained between 300 and 500 min after injection of D-Luciferin.''']] |
Revision as of 14:28, 24 September 2010
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Description
This page describes the relationship between Arabinose concentration in the medium with light output. We used a [http://www.bmglabtech.com/products/microplate-reader/instruments.cfm?product_id=2 FLUOstar OPTIMA] microplate reader to quantify the light output. Protocol and plate reader settings used are given below.
Data
Performance
| Experiment1 | Characteristic1 | Value1 |
|---|---|---|
| Transfer Function | Maximum Output | 6.6 PoPS cell-1 |
| Hill coefficient | 1.6 | |
| Switch Point | 1.5E-9 M 3OC6HSL, exogenous | |
| Response time: | <1 min | |
| Input compatibility | Strong response to | 3OC6HSL, C6HSL , C7HSL, 3OC8HSL, C8HSL |
| Weak response to | C4HSL, C10HSL, C12HSL | |
| Stability | Genetic Stability (Low/High Input) |
>92/>56 generations |
| Performance Stability (Low/High Input) |
>92/>56 generations | |
| Demand | Internal Demand (Low/High Input) |
Not measured |
| Transcriptional output demand: (Low/High Input) Nt = length of downstream transcript in nucleotides |
(0/6xNt) nucleotides cell-1 s-1 | |
| (0/1.5E-1xNt) RNAP cell-1 | ||
| Growth Rate (Low/High Input) |
54/59 min Doubling time |
1Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]
Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3
References
[http://www.ncbi.nlm.nih.gov/pubmed/18949818 [1]:] S.M. Marques and J.C.G. Esteves da Silva, (2009) Firefly Bioluminescence: A Mechanistic Approach of Luciferase Catalyzed Reactions,Life 61, 6-17.
[http://www.nature.com/nature/journal/v440/n7082/abs/nature04542.html [2]:] T. Nakatsu et al. (2006) Structural Basis for the spectral difference in luciferase bioluminescence, Nature 440(16), 372-376.
[http://www.ncbi.nlm.nih.gov/pubmed/11457857 [3]:] K. Gomi and N. Kajiyama, (2001) Oxyluciferin, a Luminescence Product of Firefly Luciferase, Is Enzymatically Regenerated into Luciferin, The Journal of Biological Chemistry, 276(39), 36508-36513.