Difference between revisions of "Part:BBa K325219"
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| − | <sup>1</sup>Measured by | + | <sup>1</sup>Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010] |
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'''Compatibility'''<br> | '''Compatibility'''<br> | ||
| − | [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell ''Chassis:''] Device has been shown to work in ''Top 10 (Invitrogen''<br> | + | [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell ''Chassis:''] Device has been shown to work in ''Top 10 (Invitrogen)''<br> |
[[Plasmid backbones|''Plasmids:'']] Device has been shown to work on ''<partinfo>pSB1C3</partinfo>'' <br> | [[Plasmid backbones|''Plasmids:'']] Device has been shown to work on ''<partinfo>pSB1C3</partinfo>'' <br> | ||
Revision as of 11:52, 24 September 2010
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Description
This part generates a red-mutant of the luciferase from the Japanese firefly (L.cruciata) as well as the luciferin regenerating enzyme (LRE). It is under the control of an Arabinose induced promoter.
D-Luciferin has to be added to obtain light output.
Performance
| Experiment1 | Characteristic1 | Value1 |
|---|---|---|
| Transfer Function | Maximum Output | 6.6 PoPS cell-1 |
| Hill coefficient | 1.6 | |
| Switch Point | 1.5E-9 M 3OC6HSL, exogenous | |
| Response time: | <1 min | |
| Input compatibility | Strong response to | 3OC6HSL, C6HSL , C7HSL, 3OC8HSL, C8HSL |
| Weak response to | C4HSL, C10HSL, C12HSL | |
| Stability | Genetic Stability (Low/High Input) |
>92/>56 generations |
| Performance Stability (Low/High Input) |
>92/>56 generations | |
| Demand | Internal Demand (Low/High Input) |
Not measured |
| Transcriptional output demand: (Low/High Input) Nt = length of downstream transcript in nucleotides |
(0/6xNt) nucleotides cell-1 s-1 | |
| (0/1.5E-1xNt) RNAP cell-1 | ||
| Growth Rate (Low/High Input) |
54/59 min Doubling time |
1Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]
Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3
References
[http://www.ncbi.nlm.nih.gov/pubmed/18949818 [1]:] S.M. Marques and J.C.G. Esteves da Silva, (2009) Firefly Bioluminescence: A Mechanistic Approach of Luciferase Catalyzed Reactions,Life 61, 6-17.
[http://www.nature.com/nature/journal/v440/n7082/abs/nature04542.html [2]:] T. Nakatsu et al. (2006) Structural Basis for the spectral difference in luciferase bioluminescence, Nature 440(16), 372-376.
[http://www.ncbi.nlm.nih.gov/pubmed/11457857 [3]:] K. Gomi and N. Kajiyama, (2001) Oxyluciferin, a Luminescence Product of Firefly Luciferase, Is Enzymatically Regenerated into Luciferin, The Journal of Biological Chemistry, 276(39), 36508-36513.