Difference between revisions of "Part:BBa K325219"
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[http://www.ncbi.nlm.nih.gov/pubmed/18949818 '''[1]:'''] S.M. Marques and J.C.G. Esteves da Silva, (2009) Firefly Bioluminescence: A Mechanistic Approach of Luciferase Catalyzed Reactions,''Life'' '''61''',6-17. | [http://www.ncbi.nlm.nih.gov/pubmed/18949818 '''[1]:'''] S.M. Marques and J.C.G. Esteves da Silva, (2009) Firefly Bioluminescence: A Mechanistic Approach of Luciferase Catalyzed Reactions,''Life'' '''61''',6-17. | ||
</div> | </div> | ||
| − | [http://www.nature.com/nature/journal/v440/n7082/abs/nature04542.html '''[2]:'''] T. Nakatsu ''et al.'' (2006) Structural Basis for the spectral difference in luciferase bioluminescence, ''Nature'' '''440''',372-376. | + | [http://www.nature.com/nature/journal/v440/n7082/abs/nature04542.html '''[2]:'''] T. Nakatsu ''et al.'' (2006) Structural Basis for the spectral difference in luciferase bioluminescence, ''Nature'' '''440'''(16),372-376. |
| + | [http://www.ncbi.nlm.nih.gov/pubmed/11457857 '''[3]:'''] K. Gomi and N. Kajiyama, (2001) Oxyluciferin, a Luminescence Product of Firefly Luciferase, Is Enzymatically Regenerated into Luciferin, ''The Journal of Biological Chemistry'', '''276'''(39),36508-36513. | ||
Revision as of 11:42, 24 September 2010
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Description
This part generates a red-mutant of the luciferase from the Japanese firefly (L.cruciata) as well as the luciferin regenerating enzyme (LRE). It is under the control of an Arabinose induced promoter.
D-Luciferin has to be added to obtain light output.
Performance
| Experiment1 | Characteristic1 | Value1 |
|---|---|---|
| Transfer Function | Maximum Output | 6.6 PoPS cell-1 |
| Hill coefficient | 1.6 | |
| Switch Point | 1.5E-9 M 3OC6HSL, exogenous | |
| Response time: | <1 min | |
| Input compatibility | Strong response to | 3OC6HSL, C6HSL , C7HSL, 3OC8HSL, C8HSL |
| Weak response to | C4HSL, C10HSL, C12HSL | |
| Stability | Genetic Stability (Low/High Input) |
>92/>56 generations |
| Performance Stability (Low/High Input) |
>92/>56 generations | |
| Demand | Internal Demand (Low/High Input) |
Not measured |
| Transcriptional output demand: (Low/High Input) Nt = length of downstream transcript in nucleotides |
(0/6xNt) nucleotides cell-1 s-1 | |
| (0/1.5E-1xNt) RNAP cell-1 | ||
| Growth Rate (Low/High Input) |
54/59 min Doubling time |
1Measured by Ania Labno and Barry Canton 2006-2007
Compatibility
Chassis: Device has been shown to work in BBa_V1000,BBa_V1001,BBa_V1002, E.Coli S30 Extract (data)
Plasmids: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430, BBa_E0434, BBa_E0240
Crosstalk with systems containing BBa_C0040.
Cell-Cell Signaling Systems: Crosstalk with devices using 3OC6HSL, C6HSL, C7HSL, C8HSL, C10HSL.
References
[http://www.ncbi.nlm.nih.gov/pubmed/18949818 [1]:] S.M. Marques and J.C.G. Esteves da Silva, (2009) Firefly Bioluminescence: A Mechanistic Approach of Luciferase Catalyzed Reactions,Life 61,6-17.
[http://www.nature.com/nature/journal/v440/n7082/abs/nature04542.html [2]:] T. Nakatsu et al. (2006) Structural Basis for the spectral difference in luciferase bioluminescence, Nature 440(16),372-376.
[http://www.ncbi.nlm.nih.gov/pubmed/11457857 [3]:] K. Gomi and N. Kajiyama, (2001) Oxyluciferin, a Luminescence Product of Firefly Luciferase, Is Enzymatically Regenerated into Luciferin, The Journal of Biological Chemistry, 276(39),36508-36513.