Difference between revisions of "Part:BBa K3893026"
Line 18: Line 18:
 
<li></html>[https://parts.igem.org/Part:BBa_K2656009 BBa_K2656009]: the [https://parts.igem.org/Part:BBa_B0030 B0030] strong ribosome biding site in its Golden Braid compatible version <html></li>
 
<li></html>[https://parts.igem.org/Part:BBa_K2656009 BBa_K2656009]: the [https://parts.igem.org/Part:BBa_B0030 B0030] strong ribosome biding site in its Golden Braid compatible version <html></li>
  
−
<li></html> [https://parts.igem.org/Part:BBa_K2656019 BBa_K2656019]: the [https://parts.igem.org/Part:BBa_K2656019 K2656013]  luxI sequence in its Golden Braid standardized version <html></li>
+
<li></html> [https://parts.igem.org/Part:BBa_K2656019 BBa_K2656019]: the [https://parts.igem.org/Part:BBa_C00161 C00161]  luxI sequence in its Golden Braid standardized version <html></li>
  
 
<li></html> Terminator  [https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version<html></li>
 
<li></html> Terminator  [https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version<html></li>

Revision as of 14:18, 16 October 2021


luxI constitutive expression

domestication.
Figure 1. SBOL Visual representation of the part


Constitutive expressed transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.

This composite part, together with K3893027, is a component of the intermediate part K3893029 to assemble the release system device (K3893030) of our Agrobactory 593. It is used to constitutively express LuxI protein, the AHL synthase, a fundamental piece of our population oscillator. release device.

This transcriptional unit is composed of the following standardized parts from Valencia_UPV 2018 iGEM Team [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

  • BBa_K2656004: the J23106 constitutive promoter in its Golden Braid compatible version
  • BBa_K2656009: the B0030 strong ribosome biding site in its Golden Braid compatible version
  • BBa_K2656019: the C00161 luxI sequence in its Golden Braid standardized version
  • Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version

Part BBa_K2656013 is the Acyl-homoserine-lactone synthase (LuxI) coding sequence C0161adapted to the Golden Braid grammar and so used with both Biobrick and Golden Gate assembly methods. It was codon-optimized for E. coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]