Difference between revisions of "Part:BBa K3893026"

Revision as of 14:13, 16 October 2021

luxI constitutive expression

Figure 1. SBOL Visual representation of the part

Constitutive expressed transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.

This composite part, together with K3893027, is a component of the intermediate part [1] to assemble the release system device ([2]) of our Agrobactory 593. It is used to constitutively express LuxI protein, the AHL synthase, a fundamental piece of our population oscillator. release device.

This transcriptional unit is composed of the following standardized parts from Valencia_UPV 2018 iGEM Team [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

• BBa_K2656004: the J23106 constitutive promoter in its Golden Braid compatible version
• BBa_K2656009: the B0030 strong ribosome biding site in its Golden Braid compatible version
• BBa_K2656019: the K2656013 luxI sequence in its Golden Braid standardized version
• Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version

Part BBa_K2656013 is the Acyl-homoserine-lactone synthase (LuxI) coding sequence C0161adapted to the Golden Braid grammar and so used with both Biobrick and Golden Gate assembly methods. It was codon-optimized for E. coli.

Sequence and Features