Difference between revisions of "Device:BBa D0001"
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'''Performance'''<br> | '''Performance'''<br> | ||
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[[Image:BBa F2620TransferFunctionOct07.png|thumb|300px|right|'''Figure 1 - Transfer function of [[BBa_D0001]]. The data points represent the mean of 6 or 9 individual measurements. The corresponding error bars represent the 95% confidence interval in the mean of the independent measurements. The blue shaded region represents the range bounded by the lowest and highest outputs among the independent measurements at a given input level. The solid black curve was calculated by fitting a simple Hill function to the experimental measurements.''']] | [[Image:BBa F2620TransferFunctionOct07.png|thumb|300px|right|'''Figure 1 - Transfer function of [[BBa_D0001]]. The data points represent the mean of 6 or 9 individual measurements. The corresponding error bars represent the 95% confidence interval in the mean of the independent measurements. The blue shaded region represents the range bounded by the lowest and highest outputs among the independent measurements at a given input level. The solid black curve was calculated by fitting a simple Hill function to the experimental measurements.''']] | ||
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*Main page | *Main page | ||
*Device Specification - experimental data and a model. Here is an example of a [http://openwetware.org/wiki/Registry_of_Standard_Biological_Models/Basic_Component_Models/Activated_Promoter model] for a biological device written in Cell ML. *User Comments and Usage Statistics | *Device Specification - experimental data and a model. Here is an example of a [http://openwetware.org/wiki/Registry_of_Standard_Biological_Models/Basic_Component_Models/Activated_Promoter model] for a biological device written in Cell ML. *User Comments and Usage Statistics | ||
| − | *Availability | + | *Availability information for physical DNA |
Latest revision as of 16:14, 11 February 2009
Designed by: Francis Crick and Jim Watson
| AHL Receiver |
|
 
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| Description | Subparts | ||||||
| BBa_D0001 is a receiver device that can be used to sense the level of a cell-cell signaling molecule 3OC6HSL. A transcription factor, LuxR (BBa_C0062) that is active in the presence of 3OC6HSL is controlled by a TetR (BBa_C0040) regulated operator (BBa_R0040). Device input is 3OC6HSL. Device output is PoPS from a LuxR-regulated operator. This device is known to work in many E. coli strains at a range of temperatures and in a variety of media types. |
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Performance
Figure 1 - Transfer function of BBa_D0001. The data points represent the mean of 6 or 9 individual measurements. The corresponding error bars represent the 95% confidence interval in the mean of the independent measurements. The blue shaded region represents the range bounded by the lowest and highest outputs among the independent measurements at a given input level. The solid black curve was calculated by fitting a simple Hill function to the experimental measurements.
| Experiment1 | Characteristic1 | Value1 |
|---|---|---|
| Transfer Function | Maximum Output | 6.6 PoPS cell-1 |
| Hill coefficient | 1.6 | |
| Switch Point | 1.5E-9 M 3OC6HSL, exogenous | |
| Response time: | <1 min | |
| Input compatibility | Strong response to | 3OC6HSL, C6HSL , C7HSL, 3OC8HSL, C8HSL |
| Weak response to | C4HSL, C10HSL, C12HSL | |
| Stability | Genetic Stability (Low/High Input) |
>92/>56 generations |
| Performance Stability (Low/High Input) |
>92/>56 generations |
Compatibility
Chassis: Device has been shown to work in BBa_V1000,BBa_V1001,BBa_V1002, E.Coli S30 Extract (data)
Plasmids: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430, BBa_E0434, BBa_E0240
Crosstalk with systems containing BBa_C0040.
Cell-Cell Signaling Systems: Crosstalk with devices using 3OC6HSL, C6HSL, C7HSL, C8HSL, C10HSL.
Pages needed for a device
- Main page
- Device Specification - experimental data and a model. Here is an example of a [http://openwetware.org/wiki/Registry_of_Standard_Biological_Models/Basic_Component_Models/Activated_Promoter model] for a biological device written in Cell ML. *User Comments and Usage Statistics
- Availability information for physical DNA