Difference between revisions of "Part:BBa K2656126"

 
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<li></html>[https://parts.igem.org/Part:BBa_K2656004 BBa_K2656004]: the [https://parts.igem.org/Part:BBa_J23106 J23106] constitutive promoter in its Golden Braid compatible version. Promoter T7 leads into a high protein expression. <html></li>
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<li></html>[https://parts.igem.org/Part:BBa_K2656004 BBa_K2656004]: the [https://parts.igem.org/Part:BBa_J23106 J23106] constitutive promoter in its Golden Braid compatible version. <html></li>
 
<li></html>[https://parts.igem.org/Part:BBa_K2656009 BBa_K2656009]: the [https://parts.igem.org/Part:BBa_B0030 B0030] ribosome biding site in its Golden Braid compatible version <html></li>
 
<li></html>[https://parts.igem.org/Part:BBa_K2656009 BBa_K2656009]: the [https://parts.igem.org/Part:BBa_B0030 B0030] ribosome biding site in its Golden Braid compatible version <html></li>
 
<li></html> [https://parts.igem.org/Part:BBa_K2656025 BBa_K2656025]: the [https://parts.igem.org/Part:BBa_J45004  BBa_J45004] BMSTI coding sequence in its Golden Braid standardized version <html></li>
 
<li></html> [https://parts.igem.org/Part:BBa_K2656025 BBa_K2656025]: the [https://parts.igem.org/Part:BBa_J45004  BBa_J45004] BMSTI coding sequence in its Golden Braid standardized version <html></li>

Latest revision as of 21:31, 17 October 2018

BMSTI transcriptional unit

Transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease (Figure 1).

TU.
Figure 1. Transcriptional unit assembled. Golden Gate scars: standardized overhangs for promoter, RBS, CDS and terminator

This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 728
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]