Difference between revisions of "Part:BBa K2656109"
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<li></html> Terminator [https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version<html></li> | <li></html> Terminator [https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version<html></li> | ||
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| + | Thus, mRFP1 sequence is assembled with a very low strength promoter and relative strong RBS. | ||
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Revision as of 22:29, 14 October 2018
mRFP1 transcriptional unit 1
Constitutive expressed transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease.
This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:
- BBa_K2656004: the J23106 constitutive promoter in its Golden Braid compatible version
- BBa_K2656009: the B0030 medium strength ribosome biding site in its Golden Braid compatible version
- BBa_K2656014: the BBa_E1010 mRFP1 sequence in its Golden Braid standardized version
- Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version
Thus, mRFP1 sequence is assembled with a very low strength promoter and relative strong RBS.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 11
Illegal NheI site found at 34 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 620
Illegal AgeI site found at 732 - 1000COMPATIBLE WITH RFC[1000]