Difference between revisions of "Part:BBa K2656014"

Revision as of 11:10, 4 October 2018

mRFP1 Coding Sequence

Part BBa_K2656014 is the monomeric Red Fluorescent Protein 1 coding secuence BBa_E1010 compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/GB3 Golden Braid 3.0] assemply methods. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol].

The characterization of this protein (and by extension of all the other part that codify for the mRFP1) has been performed with our transcriptional unit BBa_K2656109. This transcriptional unit was assembled in a Golden Braid alpha1 plasmid(REF) including the following parts:

BBa_K2656004: the J23106 promoter in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]
BBa_K2656009: the B0030 ribosome biding site in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]
BBa_K2656014: This part.
BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]

In order to carry out a correct characterization of the protein and to be able to use it to make measurements of the different transcriptional units that we have assembled with it, we have obtained the emission and excitation spectra in the conditions of our equipment. By using this protocol (REF) with the parameters of Table 1, Figure 1 has been obtained.

Parameter	Value
Number of samples	3
Excitation Wavelength measurement range 1 (nm)	[450-620]
Excitation Wavelength measurement range 2 (nm)	[620-700]
Emission wavelenght 1 (nm)	650
Emission wavelenght 2 (nm)	590
Emission Wavelength measurement range (nm)	[565-700]
Excitation wavelenght (nm)	540
Gain (G)	70
Table 1. Parameters used to obtain the spectra

Figure 1. mRFP emission and excitation spectra

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 556
Illegal AgeI site found at 668
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 Part BBa_K2656014 is the monomeric Red Fluorescent Protein 1 coding secuence [https://parts.igem.org/Part:BBa_E1010 BBa_E1010] compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/GB3 Golden Braid 3.0] assemply methods. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol].
-The characterization of this protein (and by extension of all the other part that codify for the sfGFP) has been performed with our transcriptional unit [https://parts.igem.org/Part:BBa_K2656109 BBa_K2656109].
+The characterization of this protein (and by extension of all the other part that codify for the mRFP1) has been performed with our transcriptional unit [https://parts.igem.org/Part:BBa_K2656109 BBa_K2656109].
 This transcriptional unit was assembled in a Golden Braid alpha1 plasmid'''(REF)''' including the following parts:
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