Difference between revisions of "Part:BBa I14017:Experience"
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=== Modeling crosstalk === | === Modeling crosstalk === | ||
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+ | Each experimental data set was fitted to an Hill function using the Least Absolute Residual method. | ||
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+ | [[File:ETHZ_HillEq.png|center|200px]] | ||
+ | <p>The fitting of the graphs was performed using the following equation :<br><br> | ||
+ | rFluo = the relative fluorescence (absolute measured fluorescence value over OD)[au]<br> | ||
+ | a = basal expression rate [au](“leakiness”)<br> | ||
+ | b = maximum expression rate [au]("full induction")<br> | ||
+ | n = Hill coefficient (“cooperativity”)<br> | ||
+ | K<sub>m</sub> = Half-maximal effective concentration (“sensitivity”)<br> | ||
+ | [AHL]=OHHL concentration [nM]</p> | ||
+ | <br clear="all"/> | ||
== First-order crosstalk == | == First-order crosstalk == |
Revision as of 15:46, 23 October 2014
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Applications of BBa_I14017
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ETH Zurich 2014 |
Characterization of two-order crosstalk on the promoterBackground informationSystem consideredModeling crosstalkEach experimental data set was fitted to an Hill function using the Least Absolute Residual method. The fitting of the graphs was performed using the following equation :
First-order crosstalkFirst Level crosstalk: RhlR binds to different HSL and activates the promoterSecond Level crosstalk: other regulatory proteins, like LuxR, bind to their natural HSL substrate and activates the promoterSecond order crosstalk: Combination of both cross-talk levelsOther regulatory proteins, like LuxR, bind to different HSL and activates the promoter. Results
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Antiquity |
This review comes from the old result system and indicates that this part did not work in some test. |
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