Revision as of 18:17, 20 October 2014

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Applications of BBa_C0179

User Reviews

UNIQd8cdd23b86a31243-partinfo-00000000-QINU

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ETH Zurich 2014

Characterization of two-order crosstalk

Background information

System considered

Modeling crosstalk

First-order crosstalk

First Level crosstalk: LasR binds to different HSL and activates the promoter Plas

Second Level crosstalk: LasR binds to 3OC12-HSL, its natural HSL, and activates different promoter

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Second order crosstalk: Combination of both cross-talk levels

Results

**Table 1** Crosstalk matrix for the regulator LasR (BBa_C0179)
In all the measurements conducted to create this matrix the regulator LasR was the basis and was induced in six different variations shown.

Antiquity

This review comes from the old result system and indicates that this part did not work in some test.

No review score entered. Northwestern 2011

The 2011 Northwestern iGEM team used this part as a part of our Pseudomonas Aeruginosa biosensor. We were able to successfully express RhlR (C0171) continuously in our system. BBa K575032

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iGEM Dundee 2014

Dundee iGEM 2014 used this lasR coding sequence to build two composite parts termed BBa_K1315009 and BBa_K1315010. These were designed as biosensors for Pseudomonas aeruginosa AutoInducer-1 (PAI-1), and were to be used in a bio-electronic device to improve diagnostics for Cystic Fibrosis patients. Details of experimental work are logged on the experience pages of BBa_K1315009 and BBa_K1315010.

@@ Line 51: / Line 51: @@
 |+'''Table 1''' Crosstalk matrix for the regulator LasR ([https://parts.igem.org/Part:BBa_C0179:Experience BBa_C0179])
 |colspan="4" style='font-size:10pt';text-align:left|
-In all the measurements conducted to create this matrix the [https://parts.igem.org/Part:BBa_C0062 regulator LuxR] was the basis and was induced in six different variations shown.
+In all the measurements conducted to create this matrix the [https://parts.igem.org/Part:BBa_C0179 regulator LasR] was the basis and was induced in six different variations shown.
 |-
 |style="width:200px"|[[File:ETH_Zurich_2014_qs-table_Corner_LasR.png|100px|link=https://parts.igem.org/Part:BBa_C0179:Experience]]

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