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Revision as of 18:23, 19 September 2006

A work in progress...

BBa_F2620


3OC6HSL -> PoPS Receiver

 F2620.jpg
tetR
R0040		LuxR
I0462		lux pR
R0062


Authors: Barry Canton [bcanton@mit.edu], Anna Labno [labnoa@mit.edu]                                     Last Update: Sept. 15th, 2006

Description
A transcription factor [LuxR] (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by a tetR regulatable operator (BBa_R0040. Device input is 3OC6HSL. Device output is PoPS from a LuxR-regulated operator.

Usage
Full PoPS output at high 3OC6HSL levels and high plasmid copy [e.g., pSB1A2] results in a reduced cell growth rate (see Load section). If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.

Characteristics
Input Swing: ## nM 3OC6HSL, exogenous
Output Swing: #-# PoPS
Switch Point: 2 nM 3OC6HSL, exogenous
L/H Latency: # minutes
Key Subparts
BBa_R0040: TetR-regulated operator
BBa_C0062: luxR ORF
BBa_R0062: LuxR-regulated operator


Transfer Function
 Specificity
Latency
 Stability


Demand
Transcription Demand: ## NTP per second
## polymerases sequestered
Translation Demand: # charged tRNA per second
# ribosomes sequestered
Stability
Genetic Stability: ##/## replication events (Lo Input/High Input)
Performance Stability: ##/## replication events (Lo Input/High Input)

Compatibility
Device has been shown to work in BBa_V1002, MG1655 and BBa_V1001
Device has been shown to work on pSB3K3 and pSB3K3
Device has been shown to work with BBa_E0430,BBa_E0434,BBa_E0240
Crosstalk with devices using 3OC6HSL or add in the other molecule names here. Crosstalk with systems containing TetR (BBa_C0040)
Characterization Conditions
Device output was measured using the composite part BBa_T9002, ([BBa_F2620].[BBa_E0240]). The characterization platform was BioBrick plasmidpSB3K3 with E. coli MG1655 as chassis. Cultures were grown in [http://openwetware.org/wiki/Endy:M9_media/supplemented supplemented M9 media] at 37C. Full characterization protocols can be found here.


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