Difference between revisions of "DNA Submission"

Mailing Address

Documented

Parts shipped to the Registry must be documented on the Registry.
• You must add your part to the Registry; it will then receive a part name (BBa_...)
• Your part's sequence must be entered into the Registry
• Annotate your part's sequence with features, add information on how your part works and how to use it best, and include characterization and measurement data
• Quality over quantity: sending a few well-documented parts is always better than sending several that are not
• For examples check out the collection of well documented parts.

Assembly compatible

Parts must be BioBrick (RFC 10) or iGEM Type IIS compatible
• The Registry can test and maintain all parts in the same way
• Your part can be assembled to others.
• Users can easily move/assemble the part into another plasmid backbone
• Check if your part is BioBrick or Type IIS compatible by looking at the part's sequence and feature box.
• Remember, iGEM and the Registry is built on standard biological parts!

pSB1C3

Samples must be in pSB1C3, the Registry's standard shipping backbone
• Your part sample will be flanked by the BioBrick prefix and suffix on the backbone
• The Registry can test and maintain all parts in the same way (sequence all parts using VF2 and VR primer sites).
• pSB1C3 is a high-copy plasmid, which improves miniprep yields for parts. (If your part is toxic at high copy, use pSB3C5)
• Users can easily move/assemble the part into another plasmid backbone
• You can use the pSB1C3 linearized plasmid backbone (in kit or by request) or the pSB1C3 samples in the distribution kit.