Difference between revisions of "DNA Submission"
m |
m |
||
| Line 10: | Line 10: | ||
<p>You've designed new parts, added them to the Registry and are ready to send the sample DNA. You can start a new submission, or view a previous submission and track the progress as we receive it.</p> | <p>You've designed new parts, added them to the Registry and are ready to send the sample DNA. You can start a new submission, or view a previous submission and track the progress as we receive it.</p> | ||
<div class="clear"></div> | <div class="clear"></div> | ||
| − | <div id="startSubmission" class="grid_3"><a href="" class="navbutton" style="font-size: 18px;">Start a New DNA Submission >></a></div> | + | <div id="startSubmission" class="grid_3"><a href="https://igem.org/Login" class="navbutton" style="font-size: 18px;">Login to Start a New DNA Submission >></a></div> |
| − | <div id="viewSubmission" class="grid_3"><a href="https:// | + | <div id="viewSubmission" class="grid_3"><a href="https://igem.org/Login" class="navbutton" style="font-size: 18px;">Login to View your DNA Submissions >></a></div> |
<script type="text/javascript"> | <script type="text/javascript"> | ||
| − | if(wgUserName | + | if(wgUserName != null) { |
| − | document.getElementById("startSubmission").innerHTML = '<a href=" | + | document.getElementById("startSubmission").innerHTML = '<a href="" class="navbutton" style="font-size: 18px;">Start a New DNA Submission >></a>'; |
| − | document.getElementById("viewSubmission").innerHTML = '<a href="https://igem.org/ | + | document.getElementById("viewSubmission").innerHTML = '<a href="https://parts.igem.org/cgi/dna_transfer/batch_list.cgi" class="navbutton" style="font-size: 18px;">View your DNA Submissions >></a>'; |
} | } | ||
</script> | </script> | ||
| Line 21: | Line 21: | ||
</div> | </div> | ||
<div class="grid_3" style="list-style-type: none;"> | <div class="grid_3" style="list-style-type: none;"> | ||
| − | <div class=" | + | <div class="infoBox roundCorner"> |
<h3>Mailing Address</h3> | <h3>Mailing Address</h3> | ||
<li>iGEM Foundation</li> | <li>iGEM Foundation</li> | ||
| Line 35: | Line 35: | ||
<div class="grid_4"> | <div class="grid_4"> | ||
| − | <div class=" | + | <div class="infoBox roundCorner" style="min-height: 350px;"></html> |
{{HelpPage/Submission/Document}} | {{HelpPage/Submission/Document}} | ||
<html></div> | <html></div> | ||
| Line 41: | Line 41: | ||
<div class="grid_4"> | <div class="grid_4"> | ||
| − | <div class=" | + | <div class="infoBox roundCorner" style="min-height: 350px;"></html> |
{{HelpPage/Submission/RFC10}} | {{HelpPage/Submission/RFC10}} | ||
<html></div> | <html></div> | ||
| Line 47: | Line 47: | ||
<div class="grid_4"> | <div class="grid_4"> | ||
| − | <div class=" | + | <div class="infoBox roundCorner" style="min-height: 350px;"></html> |
{{HelpPage/Submission/pSB1C3}} | {{HelpPage/Submission/pSB1C3}} | ||
<html></div> | <html></div> | ||
| Line 55: | Line 55: | ||
<div class="grid_12"> | <div class="grid_12"> | ||
| − | <div class=" | + | <div class="infoBox"> |
<h3>Preparing your DNA Shipment</a></h3> | <h3>Preparing your DNA Shipment</a></h3> | ||
<p>The Registry accepts miniprepped plasmid DNA (at least <b>10ul of 25ng/ul</b>, or 250ng total, if dried down) in <b>single PCR tubes, 8-tube strips, or 96-well plates</b>. The Registry does not accept submissions on filter paper nor does it accept live bacteria (agar stabs, glycerol, etc). Users must provide a tracking number for their shipment on the submission form, as this allows the Registry to keep track of shipments should there be customs or delivery issues.</p> | <p>The Registry accepts miniprepped plasmid DNA (at least <b>10ul of 25ng/ul</b>, or 250ng total, if dried down) in <b>single PCR tubes, 8-tube strips, or 96-well plates</b>. The Registry does not accept submissions on filter paper nor does it accept live bacteria (agar stabs, glycerol, etc). Users must provide a tracking number for their shipment on the submission form, as this allows the Registry to keep track of shipments should there be customs or delivery issues.</p> | ||
Revision as of 16:05, 23 September 2014
- Registry Help Pages:
- TOC
- At-a-Glance
- FAQ
Send a Part Sample to the Registry
You've designed new parts, added them to the Registry and are ready to send the sample DNA. You can start a new submission, or view a previous submission and track the progress as we receive it.
Mailing Address
Things to keep in mind...
Documented
- Parts shipped to the Registry must be documented on the Registry.
- You must add your part to the Registry; it will then receive a part name (BBa_...)
- Your part's sequence must be entered into the Registry
- Annotate your part's sequence with features, add information on how your part works and how to use it best, and include characterization and measurement data
- Quality over quantity: sending a few well-documented parts is always better than sending several that are not
- For examples check out the collection of well documented parts.
Assembly compatible
- Parts must be BioBrick (RFC 10) or iGEM Type IIS compatible
- The Registry can test and maintain all parts in the same way
- Your part can be assembled to others.
- Users can easily move/assemble the part into another plasmid backbone
- Check if your part is BioBrick or Type IIS compatible by looking at the part's sequence and feature box.
- Remember, iGEM and the Registry is built on standard biological parts!
pSB1C3
- Samples must be in pSB1C3, the Registry's standard shipping backbone
- Your part sample will be flanked by the BioBrick prefix and suffix on the backbone
- The Registry can test and maintain all parts in the same way (sequence all parts using VF2 and VR primer sites).
- pSB1C3 is a high-copy plasmid, which improves miniprep yields for parts. (If your part is toxic at high copy, use pSB3C5)
- Users can easily move/assemble the part into another plasmid backbone
- You can use the pSB1C3 linearized plasmid backbone (in kit or by request) or the pSB1C3 samples in the distribution kit.
Preparing your DNA Shipment
The Registry accepts miniprepped plasmid DNA (at least 10ul of 25ng/ul, or 250ng total, if dried down) in single PCR tubes, 8-tube strips, or 96-well plates. The Registry does not accept submissions on filter paper nor does it accept live bacteria (agar stabs, glycerol, etc). Users must provide a tracking number for their shipment on the submission form, as this allows the Registry to keep track of shipments should there be customs or delivery issues.
See the pages below, for more in-depth information on part submissions
DNA Submission
Sample submission of parts is no longer a requirement for the iGEM competition.
Requirements
Here's how part samples are sent to the Registry
Why pSB1C3?
Why do samples need to be shipped in pSB1C3?
Detailed Instructions
Instructions on how to fill out the Registry's submission form.
Submission Prep
Registry groups must follow iGEM's submission prep guidelines.