Difference between revisions of "Device:BBa D0001"

Revision as of 05:05, 9 January 2009

Designed by: Francis Crick and Jim Watson

AHL Receiver

Inputs: Small molecule	([3OC₆HSL])
Outputs: Transcriptional activity	(PoPS)

Description

Subparts

BBa_D0001 is a receiver device that can be used to sense the level of a cell-cell signaling molecule 3OC₆HSL. A transcription factor, LuxR (BBa_C0062) that is active in the presence of 3OC₆HSL is controlled by a TetR (BBa_C0040) regulated operator (BBa_R0040). Device input is 3OC₆HSL. Device output is PoPS from a LuxR-regulated operator. This device is known to work in many E. coli strains at a range of temperatures and in a variety of media types.

tetR R0040	LuxR I0462	lux pR R0062

Performance

Experiment¹	Characteristic¹	Value¹
Transfer Function	Maximum Output	6.6 PoPS cell^-1
	Hill coefficient	1.6
	Switch Point	1.5E-9 M 3OC₆HSL, exogenous
Response time:	<1 min
Input compatibility	Strong response to	3OC₆HSL, C₆HSL , C₇HSL, 3OC₈HSL, C₈HSL
Input compatibility	Weak response to	C₄HSL, C₁₀HSL, C₁₂HSL
Stability	Genetic Stability (Low/High Input)	>92/>56 generations
Stability	Performance Stability (Low/High Input)	>92/>56 generations

¹Measured by Ania Labno and Barry Canton 2006-2007

Compatibility
Chassis: Device has been shown to work in BBa_V1000,BBa_V1001,BBa_V1002, E.Coli S30 Extract (data)
Plasmids: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430, BBa_E0434, BBa_E0240
Crosstalk with systems containing BBa_C0040.
Cell-Cell Signaling Systems: Crosstalk with devices using 3OC₆HSL, C6HSL, C7HSL, C8HSL, C10HSL.

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What pages do we need - Main page Device Model User Comments and Usage Statistics Availability

@@ Line 6: / Line 6: @@
 |'''Subparts'''
 |-
-|BBa_D0001 is a receiver device that can be used to sense the level of a cell-cell signaling molecule [[3OC6HSL|3OC<sub>6</sub>HSL]]. A transcription factor, LuxR (''<partinfo>C0062</partinfo>'') that is active in the presence of [[3OC6HSL|3OC<sub>6</sub>HSL]] is controlled by a TetR (''<partinfo>BBa_C0040</partinfo>'') regulated operator (''<partinfo>BBa_R0040</partinfo>'').  ''Device input'' is [[3OC6HSL|3OC<sub>6</sub>HSL]].  ''Device output'' is [[PoPS]] from a LuxR-regulated operator.  If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.  This device is known to work in many ''E. coli'' strains at a range of temperatures and in a variety of media types.
+|BBa_D0001 is a receiver device that can be used to sense the level of a cell-cell signaling molecule [[3OC6HSL|3OC<sub>6</sub>HSL]]. A transcription factor, LuxR (''<partinfo>C0062</partinfo>'') that is active in the presence of [[3OC6HSL|3OC<sub>6</sub>HSL]] is controlled by a TetR (''<partinfo>BBa_C0040</partinfo>'') regulated operator (''<partinfo>BBa_R0040</partinfo>'').  ''Device input'' is [[3OC6HSL|3OC<sub>6</sub>HSL]].  ''Device output'' is [[PoPS]] from a LuxR-regulated operator.  This device is known to work in many ''E. coli'' strains at a range of temperatures and in a variety of media types.
 |<html><TABLE class='order_row' style='border: none;'border='0' cellpadding='0' cellspacing='0' color='#000000' bgcolor='#FFFFFF'><TR><TD style='font-size:90%; line-height:100%;text-align:center;'>tetR<br>R0040</TD><TD style='font-size:90%; line-height:100%;text-align:center;'>LuxR<br>I0462</TD><TD style='font-size:90%; line-height:100%;text-align:center;'>lux pR<br>R0062</TD><TR class = 'bar_row'><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=187'><img title='BBa_R0040
@@ Line 47: / Line 47: @@
 |[[Performance Stability|''Performance Stability'']]<br>(Low/High Input)
 |>92/>56 generations
-|-
-|rowspan="4"|Demand
-|rowspan="1"|Internal Demand<br>(Low/High Input)
-|Not measured
-|-
-|rowspan="2"|[[Transcription Demand|''Transcriptional output demand:'']]<br>(Low/High Input)<br>Nt = length of downstream transcript in nucleotides
-|(0/6xNt) nucleotides cell<sup>-1</sup> s<sup>-1</sup>
-|-
-|(0/1.5E-1xNt) RNAP cell<sup>-1</sup>
-|-
-|[[Growth Rate|''Growth Rate'']]<br>(Low/High Input)
-|54/59 min Doubling time
 |}
 </center>