Difference between revisions of "Part:BBa K2656013"
| Line 3: | Line 3: | ||
<partinfo>BBa_K2656013 short</partinfo> | <partinfo>BBa_K2656013 short</partinfo> | ||
| − | Part BBa_K2656013 is the superfolder Green Fluorescent Protein coding | + | Part BBa_K2656013 is the superfolder Green Fluorescent Protein coding sequence [https://parts.igem.org/Part:BBa_I746916 BBa_I746916] compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/GB3 GoldenBraid 3.0] assemply methods. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol]. |
The characterization of this protein (and by extension of all the other part that codify for the sfGFP) has been performed with our transcriptional unit [https://parts.igem.org/Part:BBa_K2656101 BBa_K2656101]. | The characterization of this protein (and by extension of all the other part that codify for the sfGFP) has been performed with our transcriptional unit [https://parts.igem.org/Part:BBa_K2656101 BBa_K2656101]. | ||
| Line 20: | Line 20: | ||
{|class='wikitable' | {|class='wikitable' | ||
| + | |colspan=4|Table 1. Parameters used to obtain the spectra | ||
| + | |- | ||
|'''Parameter''' | |'''Parameter''' | ||
|'''Value''' | |'''Value''' | ||
| Line 40: | Line 42: | ||
|Gain (G) | |Gain (G) | ||
|50 | |50 | ||
| − | |||
| − | |||
|- | |- | ||
|} | |} | ||
Revision as of 18:46, 5 October 2018
sfGFP Coding Sequence
Part BBa_K2656013 is the superfolder Green Fluorescent Protein coding sequence BBa_I746916 compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/GB3 GoldenBraid 3.0] assemply methods. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol].
The characterization of this protein (and by extension of all the other part that codify for the sfGFP) has been performed with our transcriptional unit BBa_K2656101. This transcriptional unit was assembled in a [http://2018.igem.org/Team:Valencia_UPV/GB3 Golden Braid alpha1 plasmid] including the following parts:
- BBa_K2656004: the J23106 promoter in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]
- BBa_K2656009: the B0030 ribosome biding site in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]
- BBa_K2656013: This part.
- BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]
In order to carry out a correct characterization of the protein and to be able to use it to make measurements of the different transcriptional units that we have assembled with it, we have obtained the emission and excitation spectra in the conditions of our equipment. By using this [http://2018.igem.org/Team:Valencia_UPV/Modeling#spectra protocol] with the parameters of Table 1, Figure 1 has been obtained.
| Table 1. Parameters used to obtain the spectra | |||
| Parameter | Value | ||
| Number of samples | 6 | ||
| Excitation Wavelength measurement range (nm) | [420-525] | ||
| Emission wavelenght (nm) | 545 | ||
| Emission Wavelength measurement range (nm) | [470-580] | ||
| Excitation wavelenght (nm) | 450 | ||
| Gain (G) | 50 | ||
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 14