Difference between revisions of "Frequently Used Parts"
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==Top 10 Most Used Coding Region Parts== | ==Top 10 Most Used Coding Region Parts== | ||
<parttable>Most_Used_Coding</parttable> | <parttable>Most_Used_Coding</parttable> | ||
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==Top 10 Most Used Ribosome Binding Sites== | ==Top 10 Most Used Ribosome Binding Sites== | ||
<parttable>Most_Used_RBS</parttable> | <parttable>Most_Used_RBS</parttable> | ||
− | ==Top 10 Most Used | + | |
+ | ==Top 10 Most Used Terminators== | ||
<parttable>Most_Used_Terminators</parttable> | <parttable>Most_Used_Terminators</parttable> | ||
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+ | __NOEDITSECTION__ |
Revision as of 21:58, 31 March 2014
The iGEM Registry has a vast collection of parts that have been contributed over the course of our 10 year history. We have more than 20 thousand parts that iGEMers, graduate students, postdocs, PIs and even high schoolers have contributed to the collection.
In the beginning of iGEM, it was difficult to get experiments to work as expected. As the competition matured and labs became more used to making and testing BioBricks, more things worked, but not much documentation was contributed. Finally, we required iGEMers to submit highly-documented parts to be eligible for medals. This may not seem like a big change for a single team, but we're building the Registry for future teams.
The job or every iGEMer and contributor to the Registry is to make their part as awesome as possible to inspire students to use it in following years. With over 20,000 parts already in our collection, this is not an easy feat. We encourage everybody who contributes to the Registry to complete as much information as possible and to perform as much characterization as possible before sending us a sample.
We have many ribosome binding sites in our collection. You may want a strong RBS or one for your organism of choice. However, if you're not sure, you might want one that may other people have used, will just work in . At the time of writing, our most frequently used part is BBa_b0034. This ribosome binding site, first described my Michael Elowitz in 1999 has been used over 8000 times in the history of the Registry. You can find the number of uses for each part in the part quick reference box at the top of each part page.
The purpose of this page is to showcase the most highly-used parts in our collection so you may see how iGEMers on different teams approach making great parts.
Top 10 Most Used Coding Region Parts
Name | Description | Length | Created by | Uses | Status | Doc |
---|---|---|---|---|---|---|
BBa_E0040 | green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 | 720 | jcbraff | 875 | In stock | 35565 |
BBa_E1010 | **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) | 706 | Drew Endy | 593 | In stock | 46440 |
BBa_C0040 | tetracycline repressor from transposon Tn10 (+LVA) | 685 | June Rhee, Connie Tao, Ty Thomson, Louis Waldman. | 318 | In stock | 12575 |
BBa_C0051 | cI repressor from E. coli phage lambda (+LVA) | 775 | Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross | 295 | In stock | 7093 |
BBa_C0012 | lacI repressor from E. coli (+LVA) | 1153 | Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton | 265 | In stock | 21550 |
BBa_C0062 | luxR repressor/activator, (no LVA?) | 781 | Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr | 247 | In stock | 42298 |
BBa_C0061 | autoinducer synthetase for AHL | 643 | Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr | 217 | In stock | 11645 |
BBa_E0030 | enhanced yellow fluorescent protein derived from A. victoria GFP | 723 | Caitlin Conboy and Jennifer Braff | 140 | In stock | 8152 |
BBa_I746916 | superfolder GFP coding sequence | 720 | Stefan Milde | 102 | In stock | 41280 |
BBa_K592009 | amilCP, blue chromoprotein | 669 | Lei Sun | 99 | In stock | 47318 |
Top 10 Most Used Ribosome Binding Sites
Name | Description | Length | Created by | Uses | Status | Doc |
---|---|---|---|---|---|---|
BBa_B0034 | RBS (Elowitz 1999) -- defines RBS efficiency | 12 | Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. | 5428 | In stock | 25043 |
BBa_B0030 | RBS.1 (strong) -- modified from R. Weiss | 15 | Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. | 1001 | In stock | 17415 |
BBa_B0032 | RBS.3 (medium) -- derivative of BBa_0030 | 13 | Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. | 907 | In stock | 10737 |
BBa_B0031 | RBS.2 (weak) -- derivative of BBa_0030 | 14 | Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. | 218 | In stock | 13140 |
BBa_J61100 | Ribosome Binding Site Family Member | 12 | John Anderson | 184 | In stock | 8254 |
BBa_Z0262 | Medium strength T7.2 RBS | 20 | Sriram Kosuri | 127 | Not in stock | 3181 |
BBa_J61101 | Ribosome Binding Site Family Member | 12 | John Anderson | 126 | In stock | 6164 |
BBa_Z0261 | Strong T7.2 RBS | 20 | Sriram Kosuri | 98 | Not in stock | 3179 |
BBa_K2066527 | B0034 with scar TACTAG | 18 | Joseph L Maniaci | 72 | Not in stock | 3316 |
BBa_K2406216 | MoClo RBS B0034 | 21 | Jack T. Suitor | 63 | Not in stock | 1422 |
Top 10 Most Used Terminators
Name | Description | Length | Created by | Uses | Status | Doc |
---|---|---|---|---|---|---|
BBa_B0015 | double terminator (B0010-B0012) | 129 | Reshma Shetty | 4772 | In stock | 12466 |
BBa_B0010 | T1 from E. coli rrnB | 80 | Randy Rettberg | 1064 | In stock | 11502 |
BBa_B0012 | TE from coliphageT7 | 41 | Reshma Shetty | 1021 | In stock | 5501 |
BBa_B0014 | double terminator (B0012-B0011) | 95 | Reshma Shetty | 412 | In stock | 4662 |
BBa_B1006 | Terminator (artificial, large, %T~>90) | 39 | Haiyao Huang | 221 | In stock | 1859 |
BBa_K731721 | T7 terminator | 48 | Giacomo Giacomelli, Anna Depetris | 114 | In stock | 8059 |
BBa_B0025 | double terminator (B0015), reversed | 129 | Caitlin Conboy | 102 | In stock | 4033 |
BBa_B0011 | LuxICDABEG (+/-) | 46 | Reshma Shetty | 94 | In stock | 4374 |
BBa_K2933030 | T7 terminator | 48 | Jiahao Xie | 80 | In stock | 1226 |
BBa_K2486006 | ECK120029600 - Escherichia coli K-12 terminator | 90 | Cau Westmann and Gabriel Lencioni Lovate | 77 | Not in stock | 2080 |