Difference between revisions of "Part:BBa E0030:Experience"
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The part worked as expected. The way we quantified it through a plate reader with emmission wavelengths. <br/><br/> Excitation: 515nm<br/> | The part worked as expected. The way we quantified it through a plate reader with emmission wavelengths. <br/><br/> Excitation: 515nm<br/> | ||
Emission: 528nm<br/><br/> | Emission: 528nm<br/><br/> | ||
− | We used this part for this project, data collected can be found http://2012.igem.org/Team:UIUC-Illinois/Results. | + | We used this part for this project, data collected can be found at http://2012.igem.org/Team:UIUC-Illinois/Results. |
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Revision as of 03:13, 4 October 2012
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how you used this part and how it worked out.
Applications of BBa_E0030
Characterization by British Columbia iGEM 2012
YFP fluorescence output by British Columbia iGEM 2012
The strong constitutive promoter-EYFP generator (BBa_K804001) includes an enhanced yellow fluorescence protein (BBa_E0030) under the control of a constitutive pTet promoter (BBa_J23118). This composite part's purpose is to constitutively express the YFP(BBa_E0030), which is also available with a strong ribosome binding site (BBa_B0034).
Fluorescence Output of EYFP under a pTet constituitive promoter in co-culture and mono-culture with MetA- auxotroph grown over time : MetA- auxotroph is transformed with the EYFP construct (BBa_K804001) under the constitutive pTet promoter (BBa_J23118). We co-cultured this with TyrA- auxotrophs containing a ECFP construct (BBa_K804000)under the same constitutive pTet promoter; as well as with TrpA- auxotrophs containing a RFP construct (BBa_K081012) under the same constitutive pTet promoter. These cells were grown in minimal media spiked with 10^-3M amino acids (methionine, tryptophan, and tyrosine) . Fluorescence output of EYFP for the three member co-culture, pairwise co-culture, and mono-culture with MetA- auxotrophs are analyzed using a plate reader which measures emission of YFP at a wavelength of 527nm when excited at 514nm.
User Reviews
UNIQ78474b7e98559dd7-partinfo-00000009-QINU UNIQ78474b7e98559dd7-partinfo-0000000A-QINU
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2012 iGEM UIUC |
The part worked as expected. The way we quantified it through a plate reader with emmission wavelengths. |