Part:BBa_K804001

Strong constitutive promoter-EYFP generator

This is an Enhanced Yellow Fluorescence Protein under a strong constitutive Ptet promoter (BBa_J23118). It constitutively expresses EYFP (BBa_E0430). The CFP output device does not have a LVA tag and has a strong RBS. Under a plate scanner, EYFP excites at 514nm and emits at 527nm. The fluorescence output from this construct can be used to monitor growth and population dynamics(only at exponential phase) in a microbial consortium.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]