Difference between revisions of "Part:BBa K3893026"

Latest revision as of 14:19, 16 October 2021

luxI constitutive expression

Figure 1. SBOL Visual representation of the part

Constitutive expressed transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.

This composite part, together with K3893027, is a component of the intermediate part K3893029 to assemble the release system device (K3893030) of our Agrobactory 593. It is used to constitutively express LuxI protein, the AHL synthase, a fundamental piece of our population oscillator. release device.

This transcriptional unit is composed of the following standardized parts from Valencia_UPV 2018 iGEM Team [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

BBa_K2656004: the J23106 constitutive promoter in its Golden Braid compatible version
BBa_K2656009: the B0030 strong ribosome biding site in its Golden Braid compatible version
BBa_K2656019: Codon-optimized foe E. coli, luxI sequence in its Golden Braid standardized version
Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version

Part BBa_K2656019 is the Acyl-homoserine-lactone synthase (LuxI) coding sequence C0161adapted to the Golden Braid grammar and so used with both Biobrick and Golden Gate assembly methods. It was codon-optimized for E. coli.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 11
Illegal NheI site found at 34
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3893026 short</partinfo>
-[[File:T--Ecuador--K3893026_sbolv.png|200px|thumb|right|alt=domestication.|Figure 1. SBOL Visual representation of the part ]]
+[[File:T--Ecuador--K3893026_sbolv.png|300px|thumb|right|alt=domestication.|Figure 1. SBOL Visual representation of the part ]]
 Constitutive expressed transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.
-This composite part, together with [https://parts.igem.org/Part:BBa_K3893027 K3893027], is a component of the intermediate part [https://parts.igem.org/Part:BBa_K3893028]  to assemble the release system device ([https://parts.igem.org/Part:BBa_K3893030]) of our Agrobactory 593. It is used to constitutively express LuxI protein, the AHL synthase, a fundamental piece of our population oscillator. release device.
+This composite part, together with [https://parts.igem.org/Part:BBa_K3893027 K3893027], is a component of the intermediate part [https://parts.igem.org/Part:BBa_K3893029 K3893029]  to assemble the release system device ([https://parts.igem.org/Part:BBa_K3893030 K3893030]) of our Agrobactory 593. It is used to constitutively express LuxI protein, the AHL synthase, a fundamental piece of our population oscillator. release device.
 This transcriptional unit is composed of the following standardized parts from Valencia_UPV 2018 iGEM Team [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:
@@ Line 18: / Line 18: @@
 <li></html>[https://parts.igem.org/Part:BBa_K2656009 BBa_K2656009]: the [https://parts.igem.org/Part:BBa_B0030 B0030] strong ribosome biding site in its Golden Braid compatible version <html></li>
-<li></html> [https://parts.igem.org/Part:BBa_K2656019 BBa_K2656019]: the [https://parts.igem.org/Part:BBa_K2656019 K2656013]  luxI sequence in its Golden Braid standardized version <html></li>
+<li></html> [https://parts.igem.org/Part:BBa_K2656019 BBa_K2656019]: Codon-optimized foe E. coli, luxI sequence in its Golden Braid standardized version <html></li>
 <li></html> Terminator  [https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version<html></li>
@@ Line 24: / Line 24: @@
 </html>
-Part BBa_K2656013 is the Acyl-homoserine-lactone synthase (LuxI) coding sequence [https://parts.igem.org/Part:BBa_C0161 C0161]adapted to the Golden Braid grammar and so used with both Biobrick and Golden Gate assembly methods. It was codon-optimized for E. coli.
+Part BBa_K2656019 is the Acyl-homoserine-lactone synthase (LuxI) coding sequence [https://parts.igem.org/Part:BBa_C0161 C0161]adapted to the Golden Braid grammar and so used with both Biobrick and Golden Gate assembly methods. It was codon-optimized for E. coli.