|
|
| (2 intermediate revisions by 2 users not shown) |
| Line 1: |
Line 1: |
| | + | {{HelpPage/MainLinks}} |
| | + | {{HelpPage/Learn/RelatedLinks}} |
| | + | |
| | ==Introduction== | | ==Introduction== |
| | + | The development and documentation of well-specified, standard, and interchangable biological parts is a critical step towards the design and construction of integrated biological systems. |
| | | | |
| − | The development of well-specified, standard, and interchangable biological parts is a critical step towards the design and construction of integrated biological systems. The MIT Registry of Standard Biological Parts supports this goal by recording and indexing biological parts that are currently being built and offering synthesis and assembly services to construct new parts, devices, and systems. In the future, we hope to expand this support in the areas of standards for biological part families, parameter measurement and quality control, and development of an open community of biological engineers and scientists. | + | The iGEM Registry of Standard Biological Parts is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. Parts submitted to the Registry through the iGEM competition follow the BioBricks assembly standard as described by RFC 10 and as such the vast majority of the parts in the Registry are RFC10-compatible. |
| − | | + | |
| − | ==The Registry Today==
| + | |
| − | | + | |
| − | In the summer of 2004, the Registry contained about 100 basic parts such as operators, protein coding regions, and transcriptional terminators, and devices such as logic gates built from these basic parts. Today the number of parts has increased to about 700 available parts and 2000 defined parts.
| + | |
| − | | + | |
| − | The Registry believes in the idea that a standard biological part should be well specified and able to be paired with other parts into subassemblies and whole systems. Once the parameters of these parts are determined and standardized, simulation and design of genetic systems will become easier and more reliable. The parts in the Registry are not simply segments of DNA, they are functional units. Those functions are being specified and parameters measured. Proper interoperation of a family of devices will depend on compatible parameters. For example, consideration of composable system design for gene-expression-based systems has resulted in the specification of a new unit of measurement, TIPS (TranscrIpts Per Second). TIPS measure the rate of transcription at the boundaries of a part. We are now characterizing parts in terms of TIPS. Assembly of parts into devices and systems is being performed using traditional cloning techniques with a set of restriction sites that allow easy composition of composite devies that, in turn, can themselves be used as parts. Simultaneous parallel assembly lets us build many biological systems quickly.
| + | |
| − | | + | |
| − | The Registry hosts a competition for undergraduates to challenge undergraduate students to put to use the idea that biological engineering can be made reliable through the use of standardized, well-documented parts [http://parts.mit.edu/wiki/index.php/Main_Page (iGEM)]. Many of these parts and devices already in the Registry have been developed and used by student teams to build biological systems in [http://parts.mit.edu/wiki/index.php/About_iGEM past iGEM competitions].
| + | |
| − | | + | |
| − | ==The Registry Tomorrow==
| + | |
| − | | + | |
| − | As the registry evolves, we see these key trends and challenges.
| + | |
| − | <br>
| + | |
| − | * Parameter measurement and part 'design to specification'.
| + | |
| − | * Avoidance, control and exploitation of desired or undesired evolution of parts, devices, and systems.
| + | |
| − | * Transitioning from the collection and characterization of natural parts to the design, specification, and characterization of synthetic parts.
| + | |
| − | * Developing an open and expanding community of part designers and part users.
| + | |
| − | <br>
| + | |
| − | Today, DNA synthesis is expensive; long sequence synthesis remains problematic. It is sometimes much easier to modify a natural DNA sequence than to specify and construct one from scratch. As DNA synthesis technology becomes less expensive and overcomes technical challenges, we believe that attention will shift towards DNA design (i.e., the information specifying what DNA sequence to make will be much more important than posession of a specific DNA molecule). The specification of parts and the rules for combination into systems developed for the registry will be valuable long after physical parts repositories become historical artifacts.
| + | |
| − | | + | |
| − | ==More==
| + | |
| | | | |
| − | For more information on standard assembly, biological parts, devices, vectors, and more, visit the [http://parts.mit.edu Registry]
| + | As part of the synthetic biology community's efforts to make biology easier to engineer, the Registry provides a source of genetic parts to iGEM teams during the annual competition and to academic labs through the Labs Program. Teams and other researchers are encouraged to submit their own biological parts to the Registry to help this resource stay current and grow year to year. |
The development and documentation of well-specified, standard, and interchangable biological parts is a critical step towards the design and construction of integrated biological systems.
The iGEM Registry of Standard Biological Parts is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. Parts submitted to the Registry through the iGEM competition follow the BioBricks assembly standard as described by RFC 10 and as such the vast majority of the parts in the Registry are RFC10-compatible.
As part of the synthetic biology community's efforts to make biology easier to engineer, the Registry provides a source of genetic parts to iGEM teams during the annual competition and to academic labs through the Labs Program. Teams and other researchers are encouraged to submit their own biological parts to the Registry to help this resource stay current and grow year to year.
