Difference between revisions of "Help:Reporters"
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| + | [[Category:Reporters]] | ||
[[Image:Part icon reporter.png]] | [[Image:Part icon reporter.png]] | ||
<small>Browse [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=reporter reporter parts]!</small> | <small>Browse [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=reporter reporter parts]!</small> | ||
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==Biology Background== | ==Biology Background== | ||
All of our reporter genes encode for fluorescent proteins (FP's). These fluorescent proteins are derived from two different families of proteins: the Aeqoria Victoria jellyfish, which produces a wild-type GFP, and the Discosoma corals, which (after heavy modification) produces a monomeric RFP (mRFP).<br> | All of our reporter genes encode for fluorescent proteins (FP's). These fluorescent proteins are derived from two different families of proteins: the Aeqoria Victoria jellyfish, which produces a wild-type GFP, and the Discosoma corals, which (after heavy modification) produces a monomeric RFP (mRFP).<br> | ||
| + | Common abbreviations include:<br> | ||
| + | <font color = "red">RFP</font> = red flourescent protein <br> | ||
| + | <font color = "cyan">CFP</font> = cyan flourescent protein <br> | ||
| + | <font color = "green">GFP</font> = green flourescent protein<br> | ||
| + | <font color = "yellow">YFP</font> = yellow flourescent protein<br> | ||
==Subtypes== | ==Subtypes== | ||
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===Constituitive Reporters=== | ===Constituitive Reporters=== | ||
| − | These are full [https://parts.igem.org/wiki/index.php/Add_a_Part_to_the_Registry composite parts | + | These are full [https://parts.igem.org/wiki/index.php/Add_a_Part_to_the_Registry composite parts] incorporating the Reporter Coding Region parts under constitutive [[Help:Regulatory|regulatory parts]]. These are [[Abstraction Hierarchy|devices]] which are capable of expressing proteins on their own. |
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| + | Examples of Constitutive Reporter bricks include:<br> | ||
| + | *[[Part:BBa_I13521]] - A constitutively on <font color = "red">RFP</font> coding sequence. This particular brick is also useful because it can be inactivated by the addition of tetracycline to the system. | ||
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| + | ===Inducible Reporters=== | ||
| + | The opposite of constiutive parts are called "inducible" parts, because they need the addition of a chemical or protein in order to turn on their [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Regulatory regulatory] regions.<br> | ||
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Latest revision as of 21:46, 20 July 2017
Browse reporter parts!
Contents
Biology Background
All of our reporter genes encode for fluorescent proteins (FP's). These fluorescent proteins are derived from two different families of proteins: the Aeqoria Victoria jellyfish, which produces a wild-type GFP, and the Discosoma corals, which (after heavy modification) produces a monomeric RFP (mRFP).
Common abbreviations include:
RFP = red flourescent protein
CFP = cyan flourescent protein
GFP = green flourescent protein
YFP = yellow flourescent protein
Subtypes
The Registry's reporter parts are further subdivided into the following classifications based on their
Reporter Coding Regions
These are actually parts which code for reporter proteins. On their own, these parts are NOT capable of expressing these proteins. In order to express the reporter parts listed here, you will need an additional regulatory region and a ribosome binding site preceeding this coding part.
Constituitive Reporters
These are full composite parts incorporating the Reporter Coding Region parts under constitutive regulatory parts. These are devices which are capable of expressing proteins on their own.
Examples of Constitutive Reporter bricks include:
- Part:BBa_I13521 - A constitutively on RFP coding sequence. This particular brick is also useful because it can be inactivated by the addition of tetracycline to the system.
Inducible Reporters
The opposite of constiutive parts are called "inducible" parts, because they need the addition of a chemical or protein in order to turn on their regulatory regions.
Quantification
You can find estimates of excitation wavelengths for various fluorescent proteins here.
Links
For more on our fluorescent protein parts, see the featured flourescent parts