Difference between revisions of "Part:BBa C0052"
 
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<partinfo>BBa_C0052 short</partinfo>
 
<partinfo>BBa_C0052 short</partinfo>
  
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The 434 cI repressor protein coding sequence is a 710 base-pair sequence with      the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix      sections on its ends. It binds to the 434 regulatory sequence, [[Part:BBa_R0052]].      The sequence contains a LVA tag for faster degredation and has no RBS.
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The 434 cI repressor protein coding sequence is a 710 base-pair sequence with      the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix      sections on its ends. It binds to the 434 regulatory sequence, [[Part:BBa_R0052]].      The sequence contains a LVA tag for faster degradation and has no [[Help:Ribosome Binding Site|RBS]].
  
  
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434 cI is a member of the lamboid cI protein family.
 
434 cI is a member of the lamboid cI protein family.
  
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The coding sequence has been modified from wild type cI to contain an LVA [[Help:Tag|tag]],
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The coding sequence has been modified from wild type cI to contain an LVA [[Help:Tag|tag]] and two TAA stop codons; its EcoRI recognition site has been mutated out by changing base 36 from T to C to be BioBrick-compatible.
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two TAA stop codons, and its EcoRI recognition site has been mutated out
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by changing base 36 from T to C to be BioBrick-compatible.
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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==Functional Parameters: Austin_UTexas==
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<html>
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<body>
 
<partinfo>BBa_C0052 parameters</partinfo>
 
<partinfo>BBa_C0052 parameters</partinfo>
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<!-- -->
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<h3><center>Burden Imposed by this Part:</center></h3>
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<figure>
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<div class = "center">
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<center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center>
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</div>
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<figcaption><center><b>Burden Value: 0.5 ± 2.6% </b></center></figcaption>
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</figure>
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<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the
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<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden,  and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p>
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<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
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</body>
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</html>

Latest revision as of 03:59, 26 August 2020

cI repressor from phage 434 (+LVA)

The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, Part:BBa_R0052. The sequence contains a LVA tag for faster degradation and has no RBS.


Usage and Biology

434 cI is a member of the lamboid cI protein family.

The coding sequence has been modified from wild type cI to contain an LVA tag and two TAA stop codons; its EcoRI recognition site has been mutated out by changing base 36 from T to C to be BioBrick-compatible.


Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 688
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Functional Parameters: Austin_UTexas

BBa_C0052 parameters

Burden Imposed by this Part:

Burden Value: 0.5 ± 2.6%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.