Difference between revisions of "Part:BBa K2656018"
 
(11 intermediate revisions by 2 users not shown)
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2656018 short</partinfo>
 
<partinfo>BBa_K2656018 short</partinfo>
 +
[[File:T--Valencia_UPV--im11UPV2018.png|200px|thumb|right|alt=domestication.|Figure 1. DNA basic parts domestication. Third construction corresponds with CDS Basic Part adaptation into the GoldenBraid grammar. ]]
 +
Part BBa_K2656018 is the amil Chromoprotein coding sequence [https://parts.igem.org/Part:BBa_K592009 BBa_K592009] compatible with both BioBricks and [http://2018.igem.org/Team:Valencia_UPV/Design Golden Braid 3.0] assemply methods. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the <html><a href="https://static.igem.org/mediawiki/parts/b/bc/T--Valencia_UPV--protocols.pdf#page=27"> Golden Gate assembly protocol </a></html>.
  
−
Part BBa_K592009 standarized as Phytobrick. Blue chromoprotein. It can be used to assemble transcriptional units with the Golden Gate assembly method, and also, it can be used as a Biobrick.
+
The characterization of this protein (and by extension of all the other part that codify for the amilCP) was performed with our transcriptional unit [https://parts.igem.org/Part:BBa_K2656113 BBa_K2656113].
 +
This transcriptional unit was assembled in a Golden Braid alpha 1 plasmid including the following parts:
 +
<html>
 +
<ul>
 +
<li></html>[https://parts.igem.org/Part:BBa_K2656004 BBa_K2656004]: the [https://parts.igem.org/Part:BBa_J23106 J23106] promoter in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]<html></li>
 +
<li></html>[https://parts.igem.org/Part:BBa_K2656009 BBa_K2656009]: the [https://parts.igem.org/Part:BBa_B0030 B0030] ribosome biding site in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]<html></li>
 +
<li></html>[https://parts.igem.org/Part:BBa_K2656018 BBa_K2656018]: This part.<html></li>
 +
<li></html>[https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]<html></li>
 +
</ul>
 +
</html>
 +
In order to carry out a correct characterization of the protein and to be able to use it to make measurements of the different transcriptional units that we have assembled with it, we obtained the absorbance spectrum in the conditions of our equipment. By using this [http://2018.igem.org/Team:Valencia_UPV/Experiments#spectra protocol] with the parameters of Table 1, Figure 1 was obtained.
 +
{|class='wikitable'
 +
|'''Parameter'''
 +
|'''Value'''
 +
|-
 +
|Number of samples
 +
|3
 +
|-
 +
|Wavelength measurement range (nm)
 +
|[300-700]
 +
|-
 +
|colspan=4|Table 1. Parameters used to obtain the spectum
 +
|-
 +
|}
 +
 
 +
 
 +
[[File:T--Valencia_UPV--amilCP_spectrum.png|900px|thumb|none|alt=amilCP spectum.|Figure 2. amilCP absorbance spectrum]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:21, 18 October 2018


amilCP Coding Sequence

domestication.
Figure 1. DNA basic parts domestication. Third construction corresponds with CDS Basic Part adaptation into the GoldenBraid grammar.

Part BBa_K2656018 is the amil Chromoprotein coding sequence BBa_K592009 compatible with both BioBricks and [http://2018.igem.org/Team:Valencia_UPV/Design Golden Braid 3.0] assemply methods. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the Golden Gate assembly protocol .

The characterization of this protein (and by extension of all the other part that codify for the amilCP) was performed with our transcriptional unit BBa_K2656113. This transcriptional unit was assembled in a Golden Braid alpha 1 plasmid including the following parts:

  • BBa_K2656004: the J23106 promoter in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]
  • BBa_K2656009: the B0030 ribosome biding site in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]
  • BBa_K2656018: This part.
  • BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]
In order to carry out a correct characterization of the protein and to be able to use it to make measurements of the different transcriptional units that we have assembled with it, we obtained the absorbance spectrum in the conditions of our equipment. By using this [http://2018.igem.org/Team:Valencia_UPV/Experiments#spectra protocol] with the parameters of Table 1, Figure 1 was obtained.

Parameter Value
Number of samples 3
Wavelength measurement range (nm) [300-700]
Table 1. Parameters used to obtain the spectum


amilCP spectum.
Figure 2. amilCP absorbance spectrum

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]