Difference between revisions of "Part:BBa J45320"

Latest revision as of 21:28, 16 March 2008

Salicylate generator

The salicylate generator (BBa_J45320) catalyzes the conversion of the cellular metabolite chorismate to the wintergreen odor precursor salicylate. The biosynthetic device is composed of two transcriptional devices: a constitutive transcription source (BBa_R0011) and an odor precursor enzyme generator (BBa_J45319). Odor precursor enzyme generators take as input a transcriptional signal and produce as output enzymes that catalyze production of an odor precursor from a cellular metabolite.

Usage and Biology

BBa_J45320 produces an isochorismate pyruvate-lyase and an isochorismate synthase derived from the pchBA genes from Pseudomonas aeruginosa. PchA, an isochorismate synthase, catalyzes the conversion of chorismate to isochorismate. The enzyme PchB, an isochorismate pyruvate-lyase, catalyzes the conversion of isochorismate to salicylate and pyruvate. Salicylate is the anion of salicylic acid and the two should be in rapid equilibrium. Salicylic acid is the precursor to methyl salicylate (wintergreen odor).
See BBa_J45017 for details.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 389
Illegal NgoMIV site found at 793
Illegal NgoMIV site found at 918
Illegal NgoMIV site found at 929
Illegal NgoMIV site found at 1210
Illegal NgoMIV site found at 1678
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_J45320 short</partinfo>
-Protein generator converting PoPS to the individual pchA and pchB proteins.  These two proteins biosynthesize salicylic acid. Although the coding regions overlap, there is no fusion protein; the ribosome binding site for pchA is located at the end of the coding region of pchB.  This part is almost identical to <partinfo>J45300</partinfo>, except that it uses the ribosome binding site <partinfo>BBa_B0032</partinfo> instead of <partinfo>BBa_B0030</partinfo> (which is in <partinfo>BBa_J45300</partinfo>).
+The salicylate generator (<partinfo>BBa_J45320</partinfo>) catalyzes the conversion of the cellular metabolite chorismate to the wintergreen odor precursor salicylate.  The biosynthetic device is composed of two transcriptional devices: a constitutive transcription source (<partinfo>BBa_R0011</partinfo>) and an odor precursor enzyme generator (<partinfo>BBa_J45319</partinfo>).  Odor precursor enzyme generators take as input a transcriptional signal and produce as output enzymes that catalyze production of an odor precursor from a cellular metabolite.
+[[Image:BBa J45320.png|center]]
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+*<partinfo>BBa_J45320</partinfo> produces an isochorismate pyruvate-lyase and an isochorismate synthase derived from the ''pchBA'' genes from ''Pseudomonas aeruginosa''.  PchA, an isochorismate synthase, catalyzes the conversion of chorismate to isochorismate.  The enzyme PchB, an isochorismate pyruvate-lyase, catalyzes the conversion of isochorismate to salicylate and pyruvate.  Salicylate is the anion of salicylic acid and the two should be in rapid equilibrium.  Salicylic acid is the precursor to methyl salicylate (wintergreen odor).
+*See <partinfo>BBa_J45017</partinfo> for details.
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