Difference between revisions of "Part:BBa J45320"
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The salicylic acid generator (<partinfo>BBa_J45320</partinfo>) catalyzes the conversion of the cellular metabolite chorismate to the wintergreen odor precursor salicylate. The biosynthetic device is composed of two transcriptional devices: a constitutive transcription source (<partinfo>BBa_R0011</partinfo>) and an odor precursor enzyme generator (<partinfo>BBa_J45319</partinfo>). Odor precursor enzyme generators take as input a transcriptional signal and produce as output enzymes that catalyze production of an odor precursor from a cellular metabolite. | The salicylic acid generator (<partinfo>BBa_J45320</partinfo>) catalyzes the conversion of the cellular metabolite chorismate to the wintergreen odor precursor salicylate. The biosynthetic device is composed of two transcriptional devices: a constitutive transcription source (<partinfo>BBa_R0011</partinfo>) and an odor precursor enzyme generator (<partinfo>BBa_J45319</partinfo>). Odor precursor enzyme generators take as input a transcriptional signal and produce as output enzymes that catalyze production of an odor precursor from a cellular metabolite. | ||
| − | [[Image:BBa J45320.png | + | [[Image:BBa J45320.png|center]] |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 19:33, 8 March 2008
Salicylate generator
The salicylic acid generator (BBa_J45320) catalyzes the conversion of the cellular metabolite chorismate to the wintergreen odor precursor salicylate. The biosynthetic device is composed of two transcriptional devices: a constitutive transcription source (BBa_R0011) and an odor precursor enzyme generator (BBa_J45319). Odor precursor enzyme generators take as input a transcriptional signal and produce as output enzymes that catalyze production of an odor precursor from a cellular metabolite.
Usage and Biology
- BBa_J45320 produces an isochorismate pyruvate-lyase and an isochorismate synthase derived from the pchBA genes from Pseudomonas aeruginosa. PchA, an isochorismate synthase, catalyzes the conversion of chorismate to isochorismate. The enzyme PchB, an isochorismate pyruvate-lyase, catalyzes the conversion of isochorismate to salicylate and pyruvate. Salicylic acid is the precursor to methyl salicylate (wintergreen odor).
- See BBa_J45017 for details.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 389
Illegal NgoMIV site found at 793
Illegal NgoMIV site found at 918
Illegal NgoMIV site found at 929
Illegal NgoMIV site found at 1210
Illegal NgoMIV site found at 1678 - 1000COMPATIBLE WITH RFC[1000]