Difference between revisions of "Add a Part to the Registry"
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<font color="purple">''The most important feature of a standard biological part should be that a user of the part does not have to talk to you, the designer of the part. This is achieved by completely documenting the part.''</font> | <font color="purple">''The most important feature of a standard biological part should be that a user of the part does not have to talk to you, the designer of the part. This is achieved by completely documenting the part.''</font> | ||
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Revision as of 18:26, 29 September 2017
- Registry Help Pages:
- TOC
- At-a-Glance
- FAQ
Adding Parts
Members of iGEM teams and labs may add three kinds of parts to the Registry: Basic Parts, Composite Parts, and Construction Intermediates.
Parts must have a DNA sequence. Users should document, characterize, and measure their parts, and provide that information on their part pages. Users can submit samples of their parts to the Registry.
Basic Parts
Basic Parts are discrete functional units of DNA: promoters, ribosome binding site, protein coding regions, etc. They cannot be subdivided into smaller component parts. DNA for a basic part may be obtained by ''de novo'' synthesis, by total synthesis based on a sequence from GenBank, by primer extension and PCR, or via other techniques. Like all parts submitted to the Registry, a Basic Part sample is stored in a plasmid (pSB1C3), and flanked by the BioBrick prefix and suffix. The prefix and suffix are ''not'' included in the sequence of the part when documented on the Registry. BBa_K863006 is a good example of a well documented Basic Part. Read our help page on adding a basic part
Composite Parts
Composite Parts are functional units made from an ordered series of basic parts or other composite parts. ''Explicit base pairs of DNA cannot be entered in as sequence for these parts'' (parts which do require you to manually enter sequence are Basic Parts). The Registry's software provides information and sequence for all the basic parts that you list as components of your composite part. While the Registry provides the sequence from the component parts specified, the function and design issues of the composite part should be documented in detail. BBa_K1150020 is a good example of a well documented Composite Part. Read our help page on adding a composite part.
Construction Intermediates
Construction Intermediates have no specific function and are just the result of assembling two parts together. They require no further documentation. Often they are unwanted byproducts of construction They all have the type 'Intermediate' and part names of the form 'BBa_Snnnnn'. These part names are automatically assigned by the Registry software. Once you enter your intermediate part in the Registry, you will be able to use BioBrick Blast to check your assembly's sequence and your part will show up in the subpart and superpart search functions. If you send us the DNA, we will be able to share your work with others and include it in assemblies done by the Registry.
Deleting A Part
You can try to delete a part by going to a part's "Hard Information" and setting the DNA status to "deleted".
The most important feature of a standard biological part should be that a user of the part does not have to talk to you, the designer of the part. This is achieved by completely documenting the part.
Useful Help Pages
Add a Part to the Registry: Related Registry Help Pages
- Add a Basic Part - A tutorial on how to add a basic part
- Add a Composite Part - A tutorial on how to add a composite part
- Scars - Information on assembly scars, and how to specify them for a composite part
- Synthesis - Things to keep in mind if you're adding and documenting a part you've synthesized
- Assembly Compatibility - Make sure your part is compatible with an assembly standard
- Twins - Parts are twins if they have the same sequence
- Document Parts - Recommendations on how to document your parts
- Make a Contribution - Improve existing parts
Have questions on adding a part to the Registry? Send an email to hq (at) igem . org.
FAQ
What's the difference between a basic and composite part?
- Basic Parts are discrete functional units of DNA. They cannot be subdivided into smaller component parts.
- Composite Parts are functional units made from an ordered series of basic parts or other composite parts.
In order to create a composite part, each basic part that composes it must already be added and documented to the Registry.
How do I delete a part?
You can delete a part by going to a part's "Hard Information" and setting the DNA status to "deleted".
I synthesized my entire device. Can I add it as a basic part and give it the correct part type?
No, this should be added as a composite part. You should make sure that the basic parts that compose these parts are on the Registry (already exist or you'll be adding them as new basic parts if necessary). You can can go to part tools > edit sequence and features and generate your composite part without BioBrick scars (Blunt Assembly). You can create a small basic part(s) with a part type of "Scar," and enter those in between the basic parts that constitute your composite part.
I want to add a part but do not know the sequence
All users should know their part's sequence before working with it. However, you can still add and document your part and add/edit the sequence later by going to part tools >> edit sequence and features. If you are submitting a sample of your part to the Registry, your part's sequence must be documented before submission per the Registry's submission requirements.
How do I document an improvement on an existing part on the Registry?
There are many different ways to improve an existing part, from adding measurement data to codon-optimization. Please see our help page on contributions. Note that the following page is on how to document different types of contributions or improvements. Make sure to read through the medal criteria for a given iGEM year to understand what the judges are looking for.
We have a part that we'd like to add, but it is not BioBrick RFC10 compatible or iGEM Type IIS compatible
Yes, please add and document it on the Registry. However, this part will not meet the Registry's sample submission requirements.
Should I include the BioBrick prefix and suffix or iGEM Type IIS prefix and suffix in my part's sequence?
No, your part's sequence should not include the prefix and suffix. While your parts should be assembly compatible, the prefix and suffix are an element of the plasmid backbone, not the part.