Revision as of 13:46, 24 October 2014

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Applications of BBa_C0179

User Reviews

UNIQa73da6062cc5f86c-partinfo-00000000-QINU

••••

ETH Zurich 2014

Characterization of two-order crosstalk

Background information

System considered

First-order crosstalk

First Level crosstalk: LasR binds to different HSL and activates the promoter Plas

Second Level crosstalk: LasR binds to 3OC12-HSL, its natural HSL, and activates different promoter

400px

Second order crosstalk: Combination of both cross-talk levels

Results

**Table 1** Crosstalk matrix for the regulator LasR (BBa_C0179)
In all the measurements conducted to create this matrix the regulator LasR was the basis and was induced in six different variations shown.

Antiquity

This review comes from the old result system and indicates that this part did not work in some test.

No review score entered. Northwestern 2011

The 2011 Northwestern iGEM team used this part as a part of our Pseudomonas Aeruginosa biosensor. We were able to successfully express RhlR (C0171) continuously in our system. BBa K575032

•••••

iGEM Dundee 2014

Dundee iGEM 2014 used this lasR coding sequence to build two composite parts termed BBa_K1315009 and BBa_K1315010. These were designed as biosensors for Pseudomonas aeruginosa AutoInducer-1 (PAI-1), and were to be used in a bio-electronic device to improve diagnostics for Cystic Fibrosis patients. Details of experimental work are logged on the experience pages of BBa_K1315009 and BBa_K1315010.

@@ Line 31: / Line 31: @@
 === System considered ===
-=== Modeling crosstalk ===
-Each experimental data set was fitted to an Hill function using the Least Absolute Residual method.
-[[File:ETHZ_HillEq.png|center|200px]]
-<p>The fitting of the graphs was performed using the following equation :<br><br>
-rFluo = the relative fluorescence (absolute measured fluorescence value over OD)[a.u.]<br>
-a    = basal expression rate [a.u.](“leakiness”)<br>
-b = maximum expression rate [a.u.]("full induction")<br>
-n      = Hill coefficient (“cooperativity”)<br>
-K<sub>m</sub>  = Half-maximal effective concentration (“sensitivity”)<br>
-[AHL] = AHL concentration [nM]</p>
-<br clear="all"/>
-{| border="1" class="wikitable" style="margin: 1em auto 1em auto;text-align:center;"
-|+ Parameters of Hill Function for crosstalk with LasR (with 95% confidence bounds)
-!
-! [[AHL|3OC12-HSL]]
-! [[3OC6HSL|3OC6-HSL]]
-! [[AHL|C4-HSL]]
-|-
-! [https://parts.igem.org/Part:BBa_R0079 Plas]
-|a = 5.274 (0, 15.51) [a.u.]<br> n = 11.67 (0, 3.763e8)<br> Km = 1.396 (0, 1.504e7) [nM]<br>b = 189 (162.5, 215.6) [a.u.]<br>
-| No crosstalk
-| No crosstalk
-|-
-! [https://parts.igem.org/Part:BBa_R0062 Plux]
-| a = 362.4 (158.2,566.6) [a.u.]<br> n = 0.7898 (0.3241,1.256)<br>Km = 1.269 (0, 2.548) [nM]<br> b = 1.253e4 (1.085e4, 1.422e4) [a.u.]<br>
-| No crosstalk
-| No crosstalk
-|-
-! [https://parts.igem.org/Part:BBa_I14017 Prhl]
-| a = 149.3 (140.6, 158.1) [a.u.]<br>n = 1.366 (0.808, 1.923) <br> Km = 1.674 (1.259, 2.09) [nM]<br> b = 628.9 (599, 658.7) [a.u.]<br>
-| No crosstalk
-| No crosstalk
-|}
 == First-order crosstalk ==

Difference between revisions of "Part:BBa C0179:Experience"