Part:BBa_K3740018

RBS004

Description

Modulation of protein expression level

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

2021 SZPT-China

Biology

Mutagenesis at specific position of RBS (BBa_B0034) was achieved RBS004 (BBa_K3740018) using random primers and PCR, and the constructed plasmid J23100-RBS004-sfGFP-rrnB T1 (BBa_K3740059). Compare the fluorescence intensity of sfGFP induced by J23100-B0034-sfGFP-rrnB T1 (BBa_K3740058).

Usage

Constructed J23100-RBS004-sfGFP-rrnB T1 (BBa_K3740059) was transformed into E. coli DH5α. We quantified the fluorescence intensity when the optical absorbance of bacterial culture at 600nm was around 0.2.

Characterization

The average fluorescence intensity of sfGFP induced by RBS004 (BBa_K3740018), was less than 3% of B0034 (BBa_B0034), which means reducing the pressure to the host bacteria.

Significance analysis of the average fluorescence intensity of sfGFP between B0034 and RBS004

References

[1] Zhang H M ,  Chen S ,  Shi H , et al. Measurements of Gene Expression at Steady State Improve the Predictability of Part Assembly[J]. Acs Synthetic Biology, 2016, 5(3):269.