Part:BBa_K3740010

RBS010

Description

Modulation of protein expression level

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

2021 SZPT-China

Biology

Mutagenesis at specific position of RBS (BBa_B0034) was achieved RBS010 (BBa_K3740010) using random primers and PCR, and the constructed plasmid J23100-RBS010-sfGFP-rrn B T1 (BBa_K3740046). Compare the fluorescence intensity of sfGFP induced by J23100-B0034-sfGFP-rrn B T1 (BBa_K3740058).

Usage

Constructed J23100-RBS010-sfGFP-rrn B T1 (BBa_K3740046) was transformed into E. coli DH5α. we quantified the fluorescence intensity when the optical absorbance of bacterial culture at 600nm was around 0.2.

Characterization

The average fluorescence intensity of sfGFP induced by RBS010 (BBa_K3740010), was less than 3% of B0034 (BBa_B0034), which means reducing the pressure to the host bacteria.

References

[1] Zhang H M ,  Chen S ,  Shi H , et al. Measurements of Gene Expression at Steady State Improve the Predictability of Part Assembly[J]. Acs Synthetic Biology, 2016, 5(3):269.