Part:BBa_K3425028

iGEM Type IIS pSB3C12-DY

pSB3C12-DY is a dummy sequence for iGEM Type IIS standard (RFC1000). This part mimics a multi transcriptional unit (MTU2) or an RBS basic part and can be included in the assembly whenever necessary.

This sequence should be cloned to pSB3C12 (BBa_K3425002) and used for cloning into pOdd plasmids. For the sequence ready to clone with the correct restriction sites, visit Team UofUppsala 2020's Parts page. For more information about Type IIS standard assembly, you can read Team UofUppsala 2020's Type IIS guidebook.

This part is part of iGEM Uppsala 2020's iGEM Type IIS standard collection.

The following table details the part numbers and names of other pEven dummies (which contain the plasmid they should be cloned into), along with the transcriptional unit they are mimicking.

Registry Number	Name	Mimics
BBa_K3425027	pSB3C11-DY	MTU1
BBa_K3425028	pSB3C12-DY	MTU2
BBa_K3425029	pSB3C13-DY	MTU3
BBa_K3425030	pSB3C14-DY	MTU4

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]