Plasmid_Backbone
pSB1A1

Part:pSB1A1:Design

Designed by: Reshma Shetty   Group: iGEM06_Bangalore   (2006-10-24)

pUC19 with a BioBrick cloning site (Replaced by pSB1A3)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 28
    Illegal PstI site found at 40
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2687
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal PstI site found at 40
    Illegal NotI site found at 9
    Illegal NotI site found at 2693
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2687
    Illegal BamHI site found at 22
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2687
    Illegal suffix found at 2
    Illegal XbaI site found at 28
    Illegal PstI site found at 40
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2687
    Plasmid lacks a suffix.
    Illegal XbaI site found at 28
    Illegal XbaI site found at 2702
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal PstI site found at 40
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1371
    Illegal SapI site found at 288


Design Notes

pUC19 is an extremely common cloning vector. This particular version is entered in the registry because several parts synthesized by Codon Devices (BBa_P1002, BBa_P1003, BBa_P1004, BBa_P1005, BBa_I50040) were returned in this vector. The parts were returned as the part (including BioBrick prefix and suffix) cloned between the EcoRI and BamHI site of pUC19. The BamHI has definitely been reconstituted. It is unclear whether there are 1 or 2 tandem EcoRI restriction sites upstream of the part. In this part, only a single copy of EcoRI restriction site is included. Sequence information for pUC19 can be found at http://www.fermentas.com/techinfo/nucleicacids/sequences/puc19.txt

Source

This vector was obtained from Codon Devices. Several parts synthesized by Codon Devices (BBa_P1002, BBa_P1003, BBa_P1004, BBa_P1005, BBa_I50040) were returned in this vector. Sequence information for pUC19 can be found at http://www.fermentas.com/techinfo/nucleicacids/sequences/puc19.txt

References