Part:pSB1A1

pUC19 with a BioBrick cloning site (Replaced by pSB1A3)

This plasmid is pUC19 with a BioBricks cloning site (EcoRI-NotI-XbaI-SpeI-NotI-PstI) located between the EcoRI and BamHI sites in the multiple cloning site of pUC19.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 28
  Illegal PstI site found at 40
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2687
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal PstI site found at 40
  Illegal NotI site found at 9
  Illegal NotI site found at 2693
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2687
  Illegal BamHI site found at 22
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 2687
  Illegal suffix found at 2
  Illegal XbaI site found at 28
  Illegal PstI site found at 40
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 2687
  Plasmid lacks a suffix.
  Illegal XbaI site found at 28
  Illegal XbaI site found at 2702
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal PstI site found at 40
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 1371
  Illegal SapI site found at 288