![](https://parts.igem.org/images/partbypart/icon_composite.png)
Part:BBa_K5471011
EF1a promoter infused with mir-195
This new part is a modified EF1a promoter that can drive the expression of any gene placed downstream from the promoter and simultaneously drive the transcription of a microRNA gene (https://parts.igem.org/Part:BBa_K5471009) inside the EF1a intron, which is included in the promoter (https://parts.igem.org/Part:BBa_K5471005).
This new composite part provides the ability to efficiently express a micro-RNA and at the same time express another gene of choice. This new composite part can be used for micro-RNA research, to investigate intragenic expression of microRNAs.
This composite part's sequence was used for the construction of the pAAV-EF1a-mir195-eGFP-SV40pA plasmid https://parts.igem.org/Part:BBa_K5471020 our team used for our experiments
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 452
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 452
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 600
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 452
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 452
Illegal NgoMIV site found at 335
Illegal AgeI site found at 60 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1105
None |