Composite

Part:BBa_K5471011

Designed by: Michail Diamantakis   Group: iGEM24_Patras-Med   (2024-09-29)


EF1a promoter infused with mir-195

This new part is a modified EF1a promoter that can drive the expression of any gene placed downstream from the promoter and simultaneously drive the transcription of a microRNA gene (https://parts.igem.org/Part:BBa_K5471009) inside the EF1a intron, which is included in the promoter (https://parts.igem.org/Part:BBa_K5471005).

This new composite part provides the ability to efficiently express a micro-RNA and at the same time express another gene of choice. This new composite part can be used for micro-RNA research, to investigate intragenic expression of microRNAs.

This composite part's sequence was used for the construction of the pAAV-EF1a-mir195-eGFP-SV40pA plasmid https://parts.igem.org/Part:BBa_K5471020 our team used for our experiments


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 452
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 452
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 600
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 452
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 452
    Illegal NgoMIV site found at 335
    Illegal AgeI site found at 60
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1105


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