Part:BBa_K5471009

hsa-mir-195 genomic locus (� 150bp flank)

hsa-mir-195 genomic locus (± 150bp flank) The genomic locus surrounding the gene of hsa-mir-195, spanning 150bp upstream from the 5’ end of the gene and 150bp downstream from the ‘3 end of the gene, with a combined length of 387bp (refers to the genomic locus chr17:7017465:7017851). Located in Homo Sapiens chromosome 17. Contains;

hsa-mir-195 gene: Mir-195 is contained naturally in the first intron of the polycistronic non-protein coding gene mir497hg, located in human Chromosome 17. Contains the full sequence of the micro-RNA gene, including hsa-mir-195-5p and hsa-mir-195-3p

This part is useful for anyone interested in studying the natural intragenic expression of a micro RNA in a controlled manner.

This part's sequence was used for the construction of the composite part "EF1a mir195 infuded promoter" https://parts.igem.org/Part:BBa_K5471011 which then was used for the contruction of pAAV-EF1a-mir195-eGFP-SV40pA plasmid https://parts.igem.org/Part:BBa_K5471020 our team used for our experiments

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 293