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Part:BBa_K5471009
hsa-mir-195 genomic locus (± 150bp flank)
hsa-mir-195 genomic locus (± 150bp flank) The genomic locus surrounding the gene of hsa-mir-195, spanning 150bp upstream from the 5’ end of the gene and 150bp downstream from the ‘3 end of the gene, with a combined length of 387bp (refers to the genomic locus chr17:7017465:7017851). Located in Homo Sapiens chromosome 17. Contains;
hsa-mir-195 gene: Mir-195 is contained naturally in the first intron of the polycistronic non-protein coding gene mir497hg, located in human Chromosome 17. Contains the full sequence of the micro-RNA gene, including hsa-mir-195-5p and hsa-mir-195-3p
This part is useful for anyone interested in studying the natural intragenic expression of a micro RNA in a controlled manner.
This part's sequence was used for the construction of the composite part "EF1a mir195 infuded promoter" https://parts.igem.org/Part:BBa_K5471011 which then was used for the contruction of pAAV-EF1a-mir195-eGFP-SV40pA plasmid https://parts.igem.org/Part:BBa_K5471020 our team used for our experiments
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 293
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