Coding
BisdB

Part:BBa_K123001

Designed by: Jason Gardiner   Group: iGEM08_University_of_Alberta   (2008-10-27)

BisdB

When used in conjunction with BisdA this enzyme can be used to degrade Bisphenol A. Activity has previously been recorded in E.coli.


<Bpa.jpeg>


Figure 3 BPA degradation by bisdB- and bisdAB-recombinant cells in L-BPA medium. The symbols represent the BPA content remaining in L-BPA medium in which E. coli BL21 (DE3) cells bearing pET17b (circles), pET17bisdB (triangles), and pET17bisdAB (squares) were grown. Error bars indicate standard deviations from three independent experiments. Figure Taken From: 

M. Sasaki, T. Tsuchido, Y. Matsumura. 2008 Molecular cloning and characterization of cytochrome P450 and ferredoxin 
genes involved in bisphenol A degradation in Sphingomonas bisphenolicum strain AO1. Journal of Applied Microbiology 
 105:4:1158-1169


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 479
    Illegal BamHI site found at 867
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 1276
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1086


[edit]
Categories
//function/biosynthesis
//function/degradation/bisphenol
Parameters
None