Coding

Part:BBa_K5043010

Designed by: Richard Meuten   Group: iGEM24_TUDarmstadt   (2024-09-12)


phtAd from M. vanbaalenii Pyr-1

phtAd codes for phthalate dioxygenase ferredoxin reductase. It is a subunit of ring-hydroxylating oxygenase enzyme complexes which participate in biodegradation of polycyclic aromatic hydrocarbons. [1] This part encodes for the same protein as BBa_J73043. It only differs in synonym codon usage.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 778
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 778
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 778
    Illegal XhoI site found at 1124
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 778
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 778
    Illegal NgoMIV site found at 1159
  • 1000
    COMPATIBLE WITH RFC[1000]



References

[1] S.-J. Kim, O. Kweon, R. C. Jones, J. P. Freeman, R. D. Edmondson, and C. E. Cerniglia, "Complete and integrated pyrene degradation pathway in Mycobacterium vanbaalenii PYR-1 based on systems biology," Journal of bacteriology, vol. 189, no. 2, pp. 464–472, 2007, doi: 10.1128/JB.01310-06.

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