Coding

Part:BBa_K5043007

Designed by: Richard Meuten   Group: iGEM24_TUDarmstadt   (2024-08-17)


pdoB2 from Mycobacterium sp. strain 6PY1

Encodes Phenanthrene ring-hydroxylating oxygenase, β-subunit. Presumably catalyses as a complex with pdoA2, phtAc and phtAd the oxidation of Phenanthrene-4-carboxylate to cis-phenanthrene-3,4-dihydrodiol-4-carboxylate. This is the fifth step in pyrene biodegradation. [1]

[1] S.-J. Kim, O. Kweon, R. C. Jones, J. P. Freeman, R. D. Edmondson, and C. E. Cerniglia, "Complete and integrated pyrene degradation pathway in Mycobacterium vanbaalenii PYR-1 based on systems biology," Journal of bacteriology, vol. 189, no. 2, pp. 464–472, 2007, doi: 10.1128/jb.01310-06.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 281
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None