Coding
Part:BBa_K5043007
Designed by: Richard Meuten Group: iGEM24_TUDarmstadt (2024-08-17)
pdoB2 from Mycobacterium sp. strain 6PY1
Encodes Phenanthrene ring-hydroxylating oxygenase, β-subunit. Presumably catalyses as a complex with pdoA2, phtAc and phtAd the oxidation of Phenanthrene-4-carboxylate to cis-phenanthrene-3,4-dihydrodiol-4-carboxylate. This is the fifth step in pyrene biodegradation. [1]
[1] S.-J. Kim, O. Kweon, R. C. Jones, J. P. Freeman, R. D. Edmondson, and C. E. Cerniglia, "Complete and integrated pyrene degradation pathway in Mycobacterium vanbaalenii PYR-1 based on systems biology," Journal of bacteriology, vol. 189, no. 2, pp. 464–472, 2007, doi: 10.1128/jb.01310-06.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 281
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |