Part:BBa_K3755003:Design
Mfp5 2*CDS
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 457
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence registered in this Part is a CDS without start codon or stop codon, to get the complete CDS for 2 repeats of Mfp5 protein expression, you could go to the Composite Part part:BBa_K3755012(also named as Mfp5-2). Also, if you want to design your own Mfp5 expression system or if you would like to express other proteins, the function components we have registered may be helpful(6*His tag: part:BBa_K3755020, AKTK expression tag: part:BBa_K3755009, linker containing BioBrick cut sites: part:BBa_K3755021).
Source
Isolated from Mytilus galloprovincialis cDNA library and after codon optimization
References
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