Coding
Mfp5-2

Part:BBa_K3755003:Design

Designed by: Qi Xin   Group: iGEM21_ShanghaiTech_China   (2021-09-28)


Mfp5 2*CDS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 457
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence registered in this Part is a CDS without start codon or stop codon, to get the complete CDS for 2 repeats of Mfp5 protein expression, you could go to the Composite Part part:BBa_K3755012(also named as Mfp5-2). Also, if you want to design your own Mfp5 expression system or if you would like to express other proteins, the function components we have registered may be helpful(6*His tag: part:BBa_K3755020, AKTK expression tag: part:BBa_K3755009, linker containing BioBrick cut sites: part:BBa_K3755021).

Source

Isolated from Mytilus galloprovincialis cDNA library and after codon optimization

References

[1]Danner EW, Kan Y, Hammer MU, Israelachvili JN, Waite JH. Adhesion of mussel foot protein Mefp-5 to mica: an underwater superglue. Biochemistry. 2012 Aug 21;51(33):6511-8. doi: 10.1021/bi3002538. Epub 2012 Aug 8. PMID: 22873939; PMCID: PMC3428132. [2]Yang B, Song J, Jiang Y, Li M, Wei J, Qin J, Peng W, Lasaosa FL, He Y, Mao H, Yang J, Gu Z. Injectable Adhesive Self-Healing Multicross-Linked Double-Network Hydrogel Facilitates Full-Thickness Skin Wound Healing. ACS Appl Mater Interfaces. 2020 Dec 30;12(52):57782-57797. doi: 10.1021/acsami.0c18948. Epub 2020 Dec 18. PMID: 33336572. [3]Huang J, Li H, Wang Q. [Development of double-component rapid curing bioadhesive]. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2018 Dec 25;35(6):921-927. Chinese. doi: 10.7507/1001-5515.201805045. PMID: 30583318. [4]Liang Y, Zhao X, Hu T, Han Y, Guo B. Mussel-inspired, antibacterial, conductive, antioxidant, injectable composite hydrogel wound dressing to promote the regeneration of infected skin. J Colloid Interface Sci. 2019 Nov 15;556:514-528. doi: 10.1016/j.jcis.2019.08.083. Epub 2019 Aug 24. PMID: 31473541. [5]Yang Y, Liang Y, Chen J, Duan X, Guo B. Mussel-inspired adhesive antioxidant antibacterial hemostatic composite hydrogel wound dressing via photo-polymerization for infected skin wound healing. Bioact Mater. 2021 Jun 23;8:341-354. doi: 10.1016/j.bioactmat.2021.06.014. PMID: 34541405; PMCID: PMC8427086. [6]Kord Forooshani P, Lee BP. Recent approaches in designing bioadhesive materials inspired by mussel adhesive protein. J Polym Sci A Polym Chem. 2017 Jan 1;55(1):9-33. doi: 10.1002/pola.28368. Epub 2016 Oct 11. PMID: 27917020; PMCID: PMC5132118. [7]Lee BP, Messersmith PB, Israelachvili JN, Waite JH. Mussel-Inspired Adhesives and Coatings. Annu Rev Mater Res. 2011 Aug 1;41:99-132. doi: 10.1146/annurev-matsci-062910-100429. PMID: 22058660; PMCID: PMC3207216. [8]Hwang DS, Yoo HJ, Jun JH, Moon WK, Cha HJ. Expression of functional recombinant mussel adhesive protein Mgfp-5 in Escherichia coli. Appl Environ Microbiol. 2004 Jun;70(6):3352-9. doi: 10.1128/AEM.70.6.3352-3359.2004. PMID: 15184131; PMCID: PMC427802. [9]Kim E, Dai B, Qiao JB, Li W, Fortner JD, Zhang F. Microbially Synthesized Repeats of Mussel Foot Protein Display Enhanced Underwater Adhesion. ACS Appl Mater Interfaces. 2018 Dec 12;10(49):43003-43012. doi: 10.1021/acsami.8b14890. Epub 2018 Nov 27. PMID: 30480422.