Part:BBa_K3755009

ATKA expression tag

To increase translation initiation rates for protein expression.

Usage and Biology

We learned that the first several codons are critical for efficient protein translation^[1], and the AKTK expression tag, immediately following the start codon, has been shown to increase translation initiation rates^[2].Inspired by the literature, we put AKTK expression tag after the start codon to improve the production of our protein.

Experiment and Results

Due to time reasons, we did not do the characterization experiment of AKTK increased protein expression. But we did use this basic part to produce protein with high yield. So you can see experiments and results of our Mfp5 protein production on part:BBa_K3755011 and part:BBa_K3755012.

References

[1]Tuller T, Carmi A, Vestsigian K, Navon S, Dorfan Y, Zaborske J, Pan T, Dahan O, Furman I, Pilpel Y. An evolutionarily conserved mechanism for controlling the efficiency of protein translation. Cell. 2010 Apr 16;141(2):344-54. doi: 10.1016/j.cell.2010.03.031. PMID: 20403328.

[2]Kim E, Dai B, Qiao JB, Li W, Fortner JD, Zhang F. Microbially Synthesized Repeats of Mussel Foot Protein Display Enhanced Underwater Adhesion. ACS Appl Mater Interfaces. 2018 Dec 12;10(49):43003-43012. doi: 10.1021/acsami.8b14890. Epub 2018 Nov 27. PMID: 30480422. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]