Part:BBa_K3755021

linker for mfp5 CDS&his tag

The linker connecting His tag to mfps CDS, containing BioBrick cut sites.

Usage and Biology

This is the linker we found from the literature which links 6*His tag and the protein CDS^[1]. In the supplementary material of the literature, a BioBrick cut site was mentioned and contained in this sequence, but there was no further explanation.

To find out how we used this sequence, you can go to the part:BBa_K3755011 and part:BBa_K3755012 page.

Experiment and Results

We characterized this part in larger composite parts part:BBa_K3755011 and part:BBa_K3755012, please go to that page for more details.

References

[1]Kim E, Dai B, Qiao JB, Li W, Fortner JD, Zhang F. Microbially Synthesized Repeats of Mussel Foot Protein Display Enhanced Underwater Adhesion. ACS Appl Mater Interfaces. 2018 Dec 12;10(49):43003-43012. doi: 10.1021/acsami.8b14890. Epub 2018 Nov 27. PMID: 30480422.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 16
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]