Part:BBa_K3110042:Design

lldRO1-J23117-lldRO2 Weak RBS sfGFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 78
  Illegal NheI site found at 101
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 173
  

Design Notes

This part contains:

• The Promoter and Operator Region: lldRO1-J23117-lldRO2 (Part:BBa_K1847008) -designed by iGEM team of ETH Zurich -2015
• A weak RBS: (Part:BBa_B0033)
• Reporter Protein: sfGFP (Part:BBa_I746916)
• Double terminator: (Part:BBa_I746916)

Source

sfGFP was obtained from the Biobrick. lldRO1-J23117-lldRO2+B0033 was obtained as gBlock from IDT and these were joined together using SOEing (Spliced Overlap Extenstion) PCR.

References

• parts.igem.org/Part:BBa_K1847008
• Superfolder GFP was originally described by: Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006