Composite
Part:BBa_K3044023:Design
Designed by: Catharina Bang Jensen Group: iGEM19_SDU-Denmark (2019-10-07)
dCas9 with constitutive active promotor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 312
Illegal BglII site found at 1386 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
It is important that an RBS is located between the promoter and the protein sequence in order for translation to take place.
Source
The part consists of the sequences encoding BBa_J23100, BBa_K3044008 and BBa_B0034