Composite
Kai 3
Part:BBa_K2623012
Designed by: Hongling Liu Group: iGEM18_XMU-China (2018-09-28)
SfYFP reportor for pKaiBC
SUMMARY
The sequence encoding for the gene is preceded by the specific promoter BBa_K2623009, an RBS BBa_B0034, sfYFP BBa_K864100 and followed by a double terminator BBa_B0015 in pSB1C3 to be a part of the Kai circuit, which converts periodic oscillations of protein phosphorylation into periodic fluorescent signals by periodically activated RPA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |