Composite
Kai 3

Part:BBa_K2623012

Designed by: Hongling Liu   Group: iGEM18_XMU-China   (2018-09-28)


SfYFP reportor for pKaiBC

SUMMARY

The sequence encoding for the gene is preceded by the specific promoter BBa_K2623009, an RBS BBa_B0034, sfYFP BBa_K864100 and followed by a double terminator BBa_B0015 in pSB1C3 to be a part of the Kai circuit, which converts periodic oscillations of protein phosphorylation into periodic fluorescent signals by periodically activated RPA.

Fig.1

























Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None