Coding
nosZ

Part:BBa_K1356006

Designed by: Cameron Sargent   Group: iGEM14_BYU_Provo   (2014-10-08)

Nitrous oxide reductase (nosZ) from Pseudomonas aeruginosa PAO1

This gene codes for one of the nitrous oxide reductase (nosZ) that converts nitrous oxide (N2O) into nitrogen gas (N2). This conversion is the third and final step in the denitrification pathway from nitrite (NO2-) to nitrogen gas (N2); the other genes that create this pathway can also be found in the iGEM registry (nirS (BBa_K1356003), norC (BBa_K1356004), and norB (BBa_K1356005)). A complete description of the original purpose of using this gene in reducing nitrate concentrations in the effluent of wastewater treatment facilities can be found on the BYU Provo 2014 Project Description page.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1845
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1845
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1517
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1845
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1845
    Illegal NgoMIV site found at 908
    Illegal NgoMIV site found at 1072
    Illegal AgeI site found at 282
    Illegal AgeI site found at 1381
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1638
    Illegal BsaI.rc site found at 667
    Illegal BsaI.rc site found at 1312


[edit]
Categories
//cds/biosynthesis
Parameters
None