Coding
nirS

Part:BBa_K1356003

Designed by: Cameron Sargent   Group: iGEM14_BYU_Provo   (2014-10-08)

Nitrite Reductase (nirS) from Pseudomonas aeruginosa PAO1

This gene codes for the nitrite reductase (nirS) that converts nitrite (NO2-) into nitric oxide (NO). This conversion is the first step in the denitrification pathway from nitrite (NO2-) to nitrogen gas (N2); the other genes that create this pathway can also be found in the iGEM registry (norC (BBa_K1356004), norB (BBa_K1356005), and nosZ (BBa_K1356006)). A complete description of the original purpose of using this gene in reducing nitrate concentrations in the effluent of wastewater treatment facilities can be found on the BYU Provo 2014 Project Description page.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 197
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 799
    Illegal AgeI site found at 1663
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 841
    Illegal BsaI.rc site found at 973


[edit]
Categories
//cds/biosynthesis
Parameters
None